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  • Enhanced initial cell responses to chemically modified anodized titanium.

Enhanced initial cell responses to chemically modified anodized titanium.

Clinical implant dentistry and related research (2008-02-08)
Ryo Jimbo, Takashi Sawase, Koumei Baba, Tadafumi Kurogi, Yasuaki Shibata, Mitsuru Atsuta
ABSTRACT

Previously, we reported that anodized porous titanium implants have photocatalytic hydrophilicity. However, this effect was not always sufficient for the significant improvement of bone apposition. The purpose of this study was to improve the photocatalytic properties of porous titanium implants by the fluoride modification of the anodized titanium dioxide (TiO(2)), and to investigate the initial cell response to it. The ideal concentration of ammonium hydrogen fluoride (NH(4)F-HF(2)) used in this study was determined by a static water contact angle assay. The ideal concentration of NH(4)F-HF(2) was 0.175%, and experimental disks were treated with this concentration. A pluripotent mesenchymal cell line, C2C12, was cultured on the disks in order to investigate cell attachment, morphology, and proliferation. Cell attachment after 30 minutes of culturing was significantly higher for the ultraviolet-irradiated, fluoride-modified anodized TiO(2) (p < .05), and the simultaneous scanning electron microscope observation showed a rather flattened and extended cell morphology. The proliferation rate after 24 hours was also significantly higher for the fluoride-modified anodized TiO(2). Fluoride chemical modification enhances the hydrophilic property of the anodized TiO(2) and improves the initial cell response to it.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Ammonium fluoride, ≥99.99% trace metals basis
Sigma-Aldrich
Ammonium fluoride, ACS reagent, ≥98.0%
Sigma-Aldrich
Ammonium hydrogen difluoride, reagent grade, 95%
Sigma-Aldrich
Ammonium hydrogen difluoride, 99.999% trace metals basis