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  • Standardized staining methods: Feulgen-Rossenbeck reaction for desoxyribonucleic acid and periodic acid-Schiff (PAS) procedure.

Standardized staining methods: Feulgen-Rossenbeck reaction for desoxyribonucleic acid and periodic acid-Schiff (PAS) procedure.

Biotechnic & histochemistry : official publication of the Biological Stain Commission (2002-09-17)
H O Lyon, E K Schulte, P Prento, M R Barer, M-C Béné
ABSTRACT

A project group working under the European Confederation of Laboratory Medicine (ECLM) presents recommendations for standardized procedures for the Feulgen-Rossenbeck-Schiff and the periodic acid-Schiff (PAS) reactions on cytological and histological material. The advantages and disadvantages of such standardized procedures are presented here in a preamble. Both users and manufacturers are encouraged to give their opinions with a view to achieving consensus on these procedures and on how further work on these lines may proceed.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Pararosaniline acetate, Dye content 90 %
Sigma-Aldrich
Basic Fuchsin, certified by the Biological Stain Commission, Dye content ≥88 %
Sigma-Aldrich
Basic Fuchsin, Dye content >85 %
Sigma-Aldrich
Pararosaniline hydrochloride, >85.0% (HPLC)