B6024

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside

tablet, suitable for identification of lac+ bacterial colonies

• Synonym(s): 5-Bromo-4-chloro-3-indolyl β-D-galactoside, BCIG, X-Gal
• Empirical Formula (Hill Notation): C₁₄H₁₅BrClNO₆
• CAS Number: 7240-90-6
• Molecular Weight: 408.63
• Beilstein: 1402009
• EC Number: 230-640-8
• MDL number: MFCD00005666
• UNSPSC Code: 12352204
• PubChem Substance ID: 57653950
• NACRES: NA.83

Properties

• product name: 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, tablet
• form: tablet
• suitability: suitable for identification of lac⁺ bacterial colonies
• storage temp.: −20°C
• SMILES string: OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O
• InChI: 1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1
• InChI key: OPIFSICVWOWJMJ-AEOCFKNESA-N

Description

General description

5-Bromo-4-chloro-3-indolyl β -D-galactopyranoside, commonly known as X-Gal, is a histochemical substrate for β-galactosidase.

Application

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside has been used in the preparation of X-Gal stock solution to add it in LB agar plates for blue-white screening of transformants.

X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac⁺ genotype.

Biochem/physiol Actions

X-gal is routinely used to detect transgene activity in situ.

Quantity

Contains 5 mg substrate per tablet

Reconstitution

A stock solution (100 mg/ml) is prepared by dissolving X-Gal in dimethylformamide and should be stored at −20°C and protected from light. Sterilization is not required.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves