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18909

Sigma-Aldrich

Calcofluor White Stain

suitable for microbiology

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About This Item

UNSPSC Code:
12171500
NACRES:
MA.02

product line

BioChemika

Quality Level

100

form

liquid

composition

Calcofluor White M2R, 1 g/L
Evans blue, 0.5 g/L

technique(s)

microbe id | staining: suitable

color

blue to very dark blue

λmax

423-443 nm

fluorescence

λex 423-443 nm in 0.1 M phosphate pH 7.0

application(s)

food and beverages
hematology
histology
microbiology

storage temp.

room temp

suitability

fungi
parasites
yeasts

SMILES string

C1=CC=C(C=C1)NC2=NC(=NC(=N2)N(CCO)CCO)NC3=CC(=C(C=C3)C=CC4=C(C=C(C=C4)NC5=NC(=NC(=N5)NC6=CC=CC=C6)N(CCO)CCO)S(=O)(=O)O)S(=O)(=O)O

InChI

InChI=1S/C40H44N12O10S2/c53-21-17-51(18-22-54)39-47-35(41-29-7-3-1-4-8-29)45-37(49-39)43-31-15-13-27(33(25-31)63(57,58)59)11-12-28-14-16-32(26-34(28)64(60,61)62)44-38-46-36(42-30-9-5-2-6-10-30)48-40(50-38)52(19-23-55)20-24-56/h1-16,25-26,53-56H,17-24H2,(H,57,58,59)(H,60,61,62)(H2,41,43,45,47,49)(H2,42,44,46,48,50)/b12-11+

InChI key

CNGYZEMWVAWWOB-VAWYXSNFSA-N

Related Categories

General description

Calcofluor White Stain (C.l. 40622) is a stilbene compound that has additional triazinyl and phenyl aromatic groups. It is also known as cellufluor.

Application

Calcofluor White Stain is a fluorescent stain that is used for the rapid detection of yeasts, fungi, and parasitic organisms. It is a non-specific fluorochrome that binds to cellulose and chitin in cell walls. It is suitable for use as a viability stain for plant cells. Additionally, calcofluor white is recommended as a counterstain for in situ hybridization staining of plant material and as a flow cytometry stain for assessing the viability of animal cells.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H319,H350

Hazard Classifications

Carc. 1B - Eye Irrit. 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL3756
BCCK5632
BCCK5631
BCCH2435
BCCH2434

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Bojan Gujas et al.
Development (Cambridge, England), 144(19), 3578-3589 (2017-08-31)
The plant vascular network consists of specialized phloem and xylem elements that undergo two distinct morphogenetic developmental programs to become transport-functional units. Whereas vacuolar rupture is a determinant step in protoxylem differentiation, protophloem elements never form a big central vacuole.
Melphine M Harriott et al.
Antimicrobial agents and chemotherapy, 53(9), 3914-3922 (2009-07-01)
Candida albicans readily forms biofilms on the surface on indwelling medical devices, and these biofilms serve as a source of local and systemic infections. It is estimated that 27% of nosocomial C. albicans bloodstream infections are polymicrobial, with Staphylococcus aureus
Yanqiu Zhao et al.
The New phytologist, 225(4), 1531-1544 (2019-07-02)
Wood formation is the terminal differentiation of xylem mother cells derived from cambial initials, and negative regulators play important roles in xylem differentiation. The molecular mechanism of the negative regulator of xylem differentiation PagKNAT2/6b was investigated. PagKNAT2/6b is an ortholog
Weiwei Dang et al.
Nature, 459(7248), 802-807 (2009-06-12)
Cells undergoing developmental processes are characterized by persistent non-genetic alterations in chromatin, termed epigenetic changes, represented by distinct patterns of DNA methylation and histone post-translational modifications. Sirtuins, a group of conserved NAD(+)-dependent deacetylases or ADP-ribosyltransferases, promote longevity in diverse organisms;
Derek L Lindstrom et al.
Genetics, 183(2), 413-422 (2009-08-05)
The replicative life span (RLS) of Saccharomyces cerevisiae has been established as a model for the genetic regulation of longevity despite the inherent difficulty of the RLS assay, which requires separation of mother and daughter cells by micromanipulation after every
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