- Probing a cell-embedded megadalton protein complex by DNP-supported solid-state NMR.
Probing a cell-embedded megadalton protein complex by DNP-supported solid-state NMR.
Nature methods (2015-05-20)
Mohammed Kaplan, Abhishek Cukkemane, Gydo C P van Zundert, Siddarth Narasimhan, Mark Daniëls, Deni Mance, Gabriel Waksman, Alexandre M J J Bonvin, Rémi Fronzes, Gert E Folkers, Marc Baldus
PMID25984698
ABSTRACT
Studying biomolecules at atomic resolution in their native environment is the ultimate aim of structural biology. We investigated the bacterial type IV secretion system core complex (T4SScc) by cellular dynamic nuclear polarization-based solid-state nuclear magnetic resonance spectroscopy to validate a structural model previously generated by combining in vitro and in silico data. Our results indicate that T4SScc is well folded in the cellular setting, revealing protein regions that had been elusive when studied in vitro.