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  • Effect of Argemone mexicana (L.) against lithium-pilocarpine induced status epilepticus and oxidative stress in Wistar rats.

Effect of Argemone mexicana (L.) against lithium-pilocarpine induced status epilepticus and oxidative stress in Wistar rats.

Indian journal of experimental biology (2015-02-14)
G Asuntha, Y Prasanna Raju, C R Sundaresan, Arun Rasheed, V Harini Chowdary, K R Vandana, K Satish Babu, K V S R G Prasad
ABSTRACT

Argemone mexicana (L.) has a role in the treatment of epileptic disorders in Indian traditional system of medicine. We studied its effect on induced status epilepticus (SE) and oxidative stress in rats. SE was induced in male albino rats by administration of pilocarpine (30 mg/kg, ip) 24 h after injection of lithium chloride (3 mEq/kg, ip). Different doses of the ethanol extract of A. mexicana were administered orally 1 h before the injection of pilocarpine. The severity of SE was observed and recorded every 15 min for 90 min and thereafter at every 30 min for another 90 min, using the Racine scoring system. In vivo lipid peroxidation of rat brain tissue was measured utilizing thiobarbiturate-reactive substances. Both in vitro free radical nitric oxide and 2,2-diphenyl-1-picryl hydrazyl scavenging activities of the extract were also determined. The SE severity was significantly reduced following oral administration of the extract at 250, 500 and 1000 mg/kg doses. None of the animals from groups 3 to 5 (with A. mexicana extract) have exhibited forelimb clonus of stage 4 seizure. The extract also exhibited both in vivo and in vitro antioxidant activities.

MATERIALS
Product Number
Brand
Product Description

Pilocarpine hydrochloride, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Pentylenetetrazole
Sigma-Aldrich
Pilocarpine nitrate salt, ≥98% (HPLC)
Pilocarpine nitrate for system suitability, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Pilocarpine hydrochloride, ≥99% (titration), powder
Diazepam, European Pharmacopoeia (EP) Reference Standard
Sigma-Aldrich
Diazepam
Sigma-Aldrich
Pilocarpine nitrate, meets USP testing specifications
Supelco
Diazepam solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®
Pilocarpine nitrate, European Pharmacopoeia (EP) Reference Standard
Supelco
Pilocarpine Nitrate, Pharmaceutical Secondary Standard; Certified Reference Material