Skip to Content
Merck
  • Isobutanol production from D-xylose by recombinant Saccharomyces cerevisiae.

Isobutanol production from D-xylose by recombinant Saccharomyces cerevisiae.

FEMS yeast research (2013-01-03)
Dawid Brat, Eckhard Boles
ABSTRACT

Simultaneous overexpression of an optimized, cytosolically localized valine biosynthesis pathway together with overexpression of xylose isomerase XylA from Clostridium phytofermentans, transaldolase Tal1 and xylulokinase Xks1 enabled recombinant Saccharomyces cerevisiae cells to complement the valine auxotrophy of ilv2,3,5 triple deletion mutants for growth on D-xylose as the sole carbon source. Moreover, after additional overexpression of ketoacid decarboxylase Aro10 and alcohol dehydrogenase Adh2, the cells were able to ferment D-xylose directly to isobutanol.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
2-Methyl-1-propanol, JIS special grade, ≥99.0%
Sigma-Aldrich
2-Methyl-1-propanol, SAJ first grade, ≥99.0%
Sigma-Aldrich
2-Methyl-1-propanol, anhydrous, 99.5%
Sigma-Aldrich
2-Methyl-1-propanol, BioUltra, for molecular biology, ≥99.5% (GC)
Supelco
2-Methyl-1-propanol, analytical standard
Sigma-Aldrich
2-Methyl-1-propanol, suitable for HPLC, 99.5%
Sigma-Aldrich
2-Methyl-1-propanol, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99% (GC)
Sigma-Aldrich
2-Methyl-1-propanol, 99.5%
Sigma-Aldrich
2-Methyl-1-propanol, ACS reagent, ≥99.0%
Sigma-Aldrich
Isobutyl alcohol, natural, ≥99%, FCC, FG
Sigma-Aldrich
Isobutyl alcohol, ≥99%, FCC, FG