G005721
LC/MS Analysis of Pilocarpine and Betaxolol in the Presence of Benzalkonium Chlorides on Ascentis® Express HILIC
application for HPLC
About This Item
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technique(s)
HPLC: suitable
test parameters
column: Ascentis Express HILIC, 15 cm x 4.6 mm I.D., 2.7 μm particles (53981-U)
mobile phase: [A] 2 mM arrmonium acetate, pH 6.7 in [B] 10:90 (v/v) water:acetonitrile
flow rate: 1.0 mL/min
pressure: 1160 psi (80 bar)
column temp.: 35 °C
detector: ESI(+), XlC, m/z 304, 332 (BACs), m/z 209 (pilocarpine), m/z 308 (betaxolol)
injection: 2 μL
sample: 10 μg/mL each in acetonitrile
suitability
application for HPLC
application(s)
pharmaceutical (small molecule)
Legal Information
Analyte
analytical column
standard
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