SAB4700759
IgG3 Isotype Control murine
clone PPV-07, purified immunoglobulin, buffered aqueous solution
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.42
Recommended Products
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
clone
PPV-07, monoclonal
form
buffered aqueous solution
concentration
1 mg/mL
technique(s)
flow cytometry: suitable
isotype
IgG3
shipped in
wet ice
storage temp.
2-8°C
General description
This mouse IgG3 monoclonal antibody (clone PPV-07) reacts with undefined epitope on a plant pathogen.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PPV-07, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG3 molecule and the respective sample under the particular conditions.
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PPV-07, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG3 molecule and the respective sample under the particular conditions.
Immunogen
Plum pox virus
Features and Benefits
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Physical form
Solution in phosphate buffered saline, pH 7.4, with 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Daniel Maresch et al.
Proteomics, 16(9), 1321-1330 (2016-03-10)
With mice being the top model organism in immunology and with Fc glycosylation being increasingly recognized as important modulator of antibody function, the time has come to take a look at the glycosylation of mouse IgG isotypes. Tryptic glycopeptides of
George C Brittain et al.
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 91(4), 351-363 (2017-04-04)
A biochemical system and method have been developed to enable the quantitative measurement of cytoplasmic versus nuclear localization within cells in whole blood. Compared with the analyses of nuclear localization by western blot or fluorescence microscopy, this system saves a
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