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250-05N

Sigma-Aldrich

Human Umbilical Vein Smooth Muscle Cells: HUVSMC, neonatal

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

biological source

human umbilicus (normal veins)

Quality Level

packaging

pkg of 500,000 cells

manufacturer/tradename

Cell Applications, Inc

growth mode

Adherent

karyotype

2n = 46

morphology

smooth muscle

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cardiovascular diseases

shipped in

dry ice

storage temp.

−196°C

General description

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HUVSMC has been used in studies evaluating treatments that involve blood vessel stents. Specifically, intra-shunt delivery of motexafin gadolinium was shown to inhibit the proliferation of SMCs during treatment of portal hypertension (Wang, 2013). Also, high-density lipoproteins (HDL) exhibit cardioprotective and anti-inflammatory properties by reducing HUVSMC expression of chemokines CCL2, CCL5, and CX3CL1, and chemokine receptors CCR2 and CX3CR1 (via NF-κB/pAkt inhibition) and by decreasing cell proliferation (via pERK inhibition), highlighting HDL importance in preventing neointimal hyperplasia, the main cause of early vein graft/stent failure (van der Vorst, 2013). HUVSMC were also used to demonstrate that genes coding for muscle and non-muscle actin proteins are transcribed by different transcription machinery (Lee, 2011).Characterization: positive for smooth muscle cell specific alpha-actin expression.

Cell Line Origin

Vein

Application

cell adhesion, pharmacology, vascular therapy research, coagulation, drug discovery, regulation of vascular tone

Components

Smooth Muscle Cell Basal Medium containing 10% FBS & 10% DMSO

Preparation Note

  • 2nd passage, >500,000 cells in Smooth Muscle Cell Basal Medium containing 10% FBS & 10% DMSO
  • Can be cultured at least 6 doublings

Subculture Routine

Please refer to the HUVSMC Culture Protocol.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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