11243217001
Roche
Laminin
from mouse Engelbreth-Holm-Swarm (EHS) sarcoma
Synonym(s):
Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane
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About This Item
Recommended Products
biological source
mouse (Engelbreth-Holm-Swarm (EHS) sarcoma)
Quality Level
sterility
sterile; sterile-filtered
Assay
≥90% (SDS-PAGE)
form
solution
mol wt
900 kDa
packaging
pkg of 1 mg (2 ml)
manufacturer/tradename
Roche
technique(s)
cell culture | mammalian: suitable
UniProt accession no.
storage temp.
−20°C
Gene Information
mouse ... Lamb2(16779)
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General description
Laminin is purified as laminin-nidogen complex from mouse Engelbreth-Holm-Swarm (EHS) sarcoma. Laminin is a heterotrimer made from α, β and γ chain subunits. It is a glycoprotein which is required for the structural scaffolding of basement membranes. Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells.
Biological activity: Tested for the promotion of adherence of HT-1080 cells.
Species specificity: Active on most mammalian cells.
Biological activity: Tested for the promotion of adherence of HT-1080 cells.
Species specificity: Active on most mammalian cells.
Application
Laminin has been demonstrated to promote the attachment and growth of a variety of cells; used for the coating of culture dishes. It has been used for cell adhesion assay. It is recommended for use as a cell culture substratum at 1-2μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.
Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.
Quality
Purity: ≥90% (SDS-PAGE) (as laminin nidogen-complex, 1:1)
Sequence
Mouse laminin is composed of three polypeptide chains (A: 400 kD, B1: 230 kD and B2: 220 kD), connected by disulphide bridges, and is glycosylated.
Physical form
Solution, 0.5 mg/ml laminin in 0.15 M NaCl, 2 mM EDTA, 0.05 M Tris-HCl, pH 7.4, sterile
Solution, filtered through 0.2 ?m pore size membrane
Preparation Note
Working concentration: 2 to 5 μg/cm2
2 to 5 μg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): 2 to 8 °C
The undiluted solution is stable at 2 to 8 °C for at least 3 months.
The solution should be thawed carefully at 2 to 8 °C or on ice.
2 to 5 μg/cm2 for the coating of cell culture vessels
Storage conditions (working solution): 2 to 8 °C
The undiluted solution is stable at 2 to 8 °C for at least 3 months.
The solution should be thawed carefully at 2 to 8 °C or on ice.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
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