SCM151
PLTGold® Human Platelet Lysate
Heparin-free Human Platelet Lysate (hPL) for Mesenchymal Stem Cell Culture. 100ml.
Synonym(s):
hPL, hPL Human Platelet Lysate Supplement
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About This Item
UNSPSC Code:
12352207
eCl@ss:
32160801
NACRES:
NA.81
Recommended Products
biological source
human
form
liquid
technique(s)
cell culture | stem cell: suitable
input
sample type mesenchymal stem cell(s)
shipped in
ambient
General description
PLTGold Human Platelet Lysate is a Xeno-Free cell culture supplement that is a superior alternative to fetal bovine serum (FBS) for use in human mesenchymal stem cell cultures. PLTGold is an unfractionated product derived from human platelets that does not require the addition of heparin. It remains clot-free with performance equivalent to the original PLTMax platelet lysate. Optimal growth of MSCs can be achieved with 5% PLTGold. PLTGold been used to effectively to grow mesenchymal stem cells derived from other species including mouse, rabbit and porcine.
PLTGold Human Platelet Lysate is derived from normal human donor platelets collected at U.S. blood centers. Multiple donors units are pooled in large batch sizes and manufactured to produce a consistent product. Human Platelet Lysate is for research use only (RUO) and not intended for human or animal diagnostic or therapeutic use.
PLTGold Human Platelet Lysate is derived from normal human donor platelets collected at U.S. blood centers. Multiple donors units are pooled in large batch sizes and manufactured to produce a consistent product. Human Platelet Lysate is for research use only (RUO) and not intended for human or animal diagnostic or therapeutic use.
Application
Instructions for Use
1.Cell seeding should be performed following the general guidelines for the specific cell type. For Mesenchymal Stem Cells (MSCs), cells are typically plated at approximately 2x10^3 – 5x10^3 cells per cm2.
2. For MSCs, PLTGold can be used at 5% vol/vol in a typical cell culture medium such as DMEM (Sigma D5671) or α-MEM (Sigma M8042). If the basic media doesn’t contain Glutamine, a source of L-Glutamine (Sigma G7513) will need to be added to the media at a final concentration of 2mM. For other types of cells, the concentration of PLTGold will need to be titrated according to the application (a titration from 2% vol/vol to 10% vol/vol is recommended to establish the percentage of PLTGold needed for the cell type to use).
3. Do not allow primary MSC confluence to exceed 70-80%.
1.Cell seeding should be performed following the general guidelines for the specific cell type. For Mesenchymal Stem Cells (MSCs), cells are typically plated at approximately 2x10^3 – 5x10^3 cells per cm2.
2. For MSCs, PLTGold can be used at 5% vol/vol in a typical cell culture medium such as DMEM (Sigma D5671) or α-MEM (Sigma M8042). If the basic media doesn’t contain Glutamine, a source of L-Glutamine (Sigma G7513) will need to be added to the media at a final concentration of 2mM. For other types of cells, the concentration of PLTGold will need to be titrated according to the application (a titration from 2% vol/vol to 10% vol/vol is recommended to establish the percentage of PLTGold needed for the cell type to use).
3. Do not allow primary MSC confluence to exceed 70-80%.
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Mesenchymal Stem Cells
Mesenchymal Stem Cells
Quality
All human derived materials are obtained from government inspected facilities and are of US origin. Each donor unit was approved for human use and has been tested for infectous diseases including HIV-1, HIV-2, HIV-NAT, HCV, HBsAg, and RPR for Syphilis.
Each lot is extensively tested for the following parameters:
Sterility Testing: Negative for growth
pH: 6.8-7.8
Total protein: 4.0-6.5 g/dl
Osmolality: 250-330 mOsm/kg
Endotoxin USP <8>: <10 EU/mL
Mycoplasma: Not detected
Cell Growth: Pass
Each lot is extensively tested for the following parameters:
Sterility Testing: Negative for growth
pH: 6.8-7.8
Total protein: 4.0-6.5 g/dl
Osmolality: 250-330 mOsm/kg
Endotoxin USP <8>: <10 EU/mL
Mycoplasma: Not detected
Cell Growth: Pass
Storage and Stability
Store at -80°C upon receipt. Thaw at room temperature or in a water bath. After thawing, aliquot and store at -20°C or colder. Long term storage at -80°C is recommended. Multiple freeze/thaw cycles are not recommended.
Legal Information
PLTGold is a registered trademark of Mill Creek Life Sciences, LLC
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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