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biological source
human
Quality Level
Assay
>98%
form
liquid
manufacturer/tradename
Chemicon®
NCBI accession no.
UniProt accession no.
shipped in
dry ice
General description
High Density Lipoprotein (HDL) isolated by sequential isopycnic ultracentrifugation are delipidated. Apo A-II is purified by column chromatography in 6M urea (Schonfeld et al. 1977). Purity is determined by quantitative densitometry of SDS polyacrylamide gels (Laemlli 1970). Protein is determined by the Pierce BCA method (which was found to be comparable to the Lowry method) with 1% BSA as the standard. Apo A-II, which is a dimer composed of identical subunits, has a Mr of 17 kD and a pI of 4.9. Apo-II activates hepatic lipase and inhibits LCAT. Apolipoproteins are soluble in aqueous solutions. However, due to its amphipathic structure Apo-II self-associates into oligomers at high protein concentrations. Apo A-II exists as dimers in buffers with 5M guanidine-HCL or 6M urea.
MOLECULAR WEIGHT:
17,400
MOLECULAR WEIGHT:
17,400
Product Source: Normal human plasma, fresh, non-frozen. Tested negative for HIV-1, HIV-2, HCV and HBc antibodies and HBsAg.
Physical form
Liquid in 10 mM NH4HCO3, pH 7.4. Non-sterile, no preservatives.
Storage and Stability
Maintain lyophilized material at < -20°C for up to six months. Maintain reconstituted Apo A-II at +4°C for up to 4 weeks. Freezing solutions of apolipoproteins in the absence of denaturants may favor increased self-association or aggregation.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Solubilization of low-density lipoprotein with sodium deoxycholate and recombination of apoprotein B with dimyristoylphosphatidylcholine.
Walsh, M T and Atkinson, D
Biochemistry, 22, 3170-3178 (1983)
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