Murashige and Skoog Plant Cell Culture Media Formulations

Much of plant tissue culture research today still utilizes media developed many years ago by Murashige and Skoog. Below you will find formulation information for many of these media.

• Basal Salts Mixtures
• Media including Organics
  

Basal Salts Mixtures

M5524 – Murashige and Skoog basal salt mixture (MS)
M0654 – Murashige and Skoog basal salt macronutrient solution
M0529 – Murashige and Skoog basal salt micronutrient solution
M2909 – Murashige and Skoog modified basal salt mixture

Component (mg/L)	M5524	M0654	M0529	M2909
Aluminum chloride • 6H2O
Ammonium nitrate	1650.0	1650.0
Ammonium phosphate monobasic
Ammonium sulfate
Boric acid	6.2		6.2	6.2
Calcium chloride anhydrous	332.2	332.2		332.2
Calcium nitrate
Calcium phosphate tribasic
Cobalt chloride • 6H2O	0.025		0.025	0.025
Cupric sulfate • 5H2O	0.025		0.025	0.025
Na2-EDTA	37.26		37.3	37.26
ZnNa2-EDTA
Ferric chloride • 6H2O
Ferric citrate
Ferric sulfate
Ferric tartrate • 2H2O
Ferrous sulfate • 7H2O	27.8		27.8	27.8
Magnesium sulfate	180.7	180.7		180.7
Manganese chloride • 4H2O
Manganese sulfate • H2O	16.9		16.9	16.9
Molybdenum trioxide
Molybdic acid (sodium salt) • 2H2O	0.25		0.25	0.25
Nickel chloride • 6H2O
Nickel sulfate • 6H2O
Potassium chloride
Potassium iodide	0.83		0.83	0.83
Potassium nitrate	1900.0	1900.0		1900.0
Potassium phosphate monobasic	170.0	170.0		170.0
Potassium sulfate
Sodium nitrate
Sodium phosphate monobasic
Sodium sulfate
Zinc nitrate • 6H2O
Zinc sulfate • 7H2O	8.6		8.6	8.6

Organics (mg/L)	M5524	M0654	M0529	M2909
Activated charcoal
Adenine hemisulfate
Agar
6-Benzylaminopurine (BA)
D-Biotin
6gamma-gamma- Dimethylallylaminopurine
Glycine (free base)
Indole-3-acetic acid
Indole-3-butyric acid
Kinetin
MES (free acid)
myo-Inositol
alpha-Naphthaleneacetic acid
Nictotinic acid (free acid)
Peptone
Pyridoxine • HCl
Sucrose
Thiamine • HCl

	M5524	M0654	M0529	M2909
Grams of powder to prepare 1 L	4.3	n/a	n/a	2.7
pH ± 0.5 at room temperature	3.9	4.3	3.2	3.9

Media including Organics

M6899 – Murashige and Skoog basal salts with minimal organics
M5519 – Murashige and Skoog basal medium
M0404 – Murashige and Skoog basal medium with Gamborg's vitamins
M9274 – Murashige and Skoog basal medium with sucrose and agar
M7024 – Murashige and Skoog shoot multiplication medium A

Component (mg/L)	M6899	M5519	M0404	M9274	M7024
Aluminum chloride • 6H2O
Ammonium nitrate	1650.0	1650.0	1650.0	1650.0	1650.0
Ammonium phosphate monobasic
Ammonium sulfate
Boric acid	6.2	6.2	6.2	6.2	6.2
Calcium chloride anhydrous	332.2	332.2	332.2	332.2	332.2
Calcium nitrate
Calcium phosphate tribasic
Cobalt chloride • 6H2O	0.025	0.025	0.025	0.025	0.025
Cupric sulfate • 5H2O	0.025	0.025	0.025	0.025	0.025
Na2-EDTA	37.26	37.26	37.26	37.26	37.26
ZnNa2-EDTA
Ferric chloride • 6H2O
Ferric citrate
Ferric sulfate
Ferric tartrate • 2H2O
Ferrous sulfate • 7H2O	27.8	27.8	27.8	27.8	27.8
Magnesium sulfate	180.7	180.7	180.7	180.7	180.7
Manganese chloride • 4H2O
Manganese sulfate • H2O	16.9	16.9	16.9	16.9	16.9
Molybdenum trioxide
Molybdic acid (sodium salt) • 2H2O	0.25	0.25	0.25	0.25	0.25
Nickel chloride • 6H2O
Nickel sulfate • 6H2O
Potassium chloride
Potassium iodide	0.83	0.83	0.83	0.83	0.83
Potassium nitrate	1900.0	1900.0	1900.0	1900.0	1900.0
Potassium phosphate monobasic	170.0	170.0	170.0	170.0	170.0
Potassium sulfate
Sodium nitrate
Sodium phosphate monobasic					148.0
Sodium sulfate
Zinc nitrate • 6H2O
Zinc sulfate • 7H2O	8.6	8.6	8.6	8.6	8.6

Organics (mg/L)	M6899	M5519	M0404	M9274	M7024
Activated charcoal
Adenine hemisulfate					80.0
Agar				8000.0
6-Benzylaminopurine (BA)
D-Biotin
6gamma-gamma- Dimethylallylaminopurine					30.0
Glycine (free base)		2.0		2.0
Indole-3-acetic acid					0.3
Indole-3-butyric acid
Kinetin
MES (free acid)
myo-Inositol	100.0	100.0	100.0	100.0	100.0
alpha-Naphthaleneacetic acid
Nictotinic acid (free acid)		0.5	1.0	0.5
Peptone
Pyridoxine • HCl		0.5	1.0	0.5
Sucrose				30,000	30,000
Thiamine • HCl	0.4	0.1	10.0	0.1	0.4

	M6899	M5519	M0404	M9274	M7024
Grams of powder to prepare 1 L	4.4	4.4	4.4	42.4	34.7
pH ± 0.5 at room temperature	3.9	3.9	3.8	5.0	3.9