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HPLC Analysis of Triazole Antifungal Agent Enantiomers on Astec® CYCLOBOND I 2000 HP-RSP

HPLC Analysis of Triazole Antifungal Agent Enantiomers on Astec® CYCLOBOND I 2000 HP-RSP application for HPLC

Materiales

analytical column

Referencia del producto
Descripción
Precios

24024AST

Astec® Cyclobond I 2000 HP-RSP Chiral HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

standard

Referencia del producto
Descripción
Precios

K1003

Ketoconazole

99.0-101.0% (EP, titration)

M3512

(±)-Miconazole nitrate salt

imidazole antibiotic

UC280

Ketoconazole

CONDITIONS

column

Astec CYCLOBOND I 2000 HP-RSP, 25 cm x 4.6 mm I.D., 5 μm particles (24024AST)

mobile phase

[A] 0.1% formic acid; [B] acetonitrile; (70:30, A:B)

flow rate

1 mL/min

detector

UV, 230 nm

injection

1 μL

sample

0.1 mg/mL in methanol
peaks 1&2: enilconazole
peaks 2&3: eberconazole
peaks 4&5: miconazole
peaks 6&7: ketoconazole

Descripción

Analysis Note

Note: Peak 2 of enilconzaole coelutes with peak 1 of eberconazole under these conditions.

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
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