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HPLC Analysis of Spice Cannabinoids on Ascentis® Express C18

HPLC Analysis of Spice Cannabinoids on Ascentis® Express C18 application for HPLC

Materiales

analytical column

Referencia del producto
Descripción
Precios

Columna para HPLC Ascentis® Express C18, 2,7 μm

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

standard

Referencia del producto
Descripción
Precios

Spice Cannabinoid Mix solution

100 μg/mL each component in acetonitrile, ampule of 1.0 mL, certified reference material, Cerilliant®

HU-210 solution

100 μg/mL in methanol, (Spice Cannabinoid), ampule of 1 mL, certified reference material, Cerilliant®

JWH-018 solution

100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

JWH-073 solution

100 μg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

JWH-073 N-(3-hydroxybutyl) metabolite solution

100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)

mobile phase

[A] 5 mM ammonium formate water; [B] 5 mM ammonium formate (95:5, acetonitrile:water)

gradient

68 to 100% B in 8 min; held at 100% B for 2 min

flow rate

0.6 mL/min

pressure

4351 psi (300 bar)

column temp.

30 °C

detector

UV, 265 nm

injection

3 μL

Descripción

Analysis Note

Synthetic cannabinoids (e.g. "Spice") are a type of designer drug used to get a cannabis-type high. New synthetic cannabinoids are continually being introduced as suppliers tweak the molecular structures. The ability to rapidly and reliably identify the continually changing population of these compounds in the blood or urine suspected users is a significant analytical challenge facing forensic chemists. Here is shown the selectivity provided by C18 phase chemistry on an Ascentis Express C18 column with UV detection. Cerilliant CRMs provided reliable identification and quantification.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany