HPLC Analysis of Ginsenosides on Ascentis^® Express C18

column

Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 μm particles (53829-U)

mobile phase

[A] water; [B] acetonitrile

gradient

25% B for 1.5 min; 25 to 85% B in 12 min; hold at 85% B for 1 min

flow rate

1 mL/min

column temp.

35 °C

detector

UV, 254 nm

injection

5 μL

sample

50 μg/mL in 82:18, water:methanol