HPLC Analysis of Bupivacaine Enantiomers on Astec^® CHIROBIOTIC^® V2, Formate Mobile Phase

column

CHIROBIOTIC V2, 25 cm x 4.6 mm I.D., 5 μm particles (15024AST)

mobile phase

[A] 20 mM ammonium formate, pH 4.1; [B] methanol; (5:95, A:B)

flow rate

1 mL/min

column temp.

20 °C

detector

UV, 220 nm

injection

5 μL

sample

1 mg/mL in methanol