Saltar al contenido
Merck
  • The short-term toxic effects of TiO₂nanoparticles toward bacteria through viability, cellular respiration, and lipid peroxidation.

The short-term toxic effects of TiO₂nanoparticles toward bacteria through viability, cellular respiration, and lipid peroxidation.

Environmental science and pollution research international (2015-07-15)
Ayca Erdem, David Metzler, Daniel K Cha, C P Huang
RESUMEN

To better understand the potential impacts of metal oxide nanoparticles (NPs) on Gram(+) Bacillus subtilis and Gram(-) Escherichia coli (K12) bacteria, eight different nanosized titanium dioxide (TiO2) suspensions with five different concentrations were used. Water quality parameters (pH, temperature, and ionic strength), light sources, and light intensities were also changed to achieve different environmental conditions. The photosensitive TiO2 NPs were found to be harmful to varying degrees under ambient conditions, with antibacterial activity increasing with primary particle sizes from 16 to 20 nm. The presence of light was a significant factor under most conditions tested, presumably due to its role in promoting generation of reactive oxygen species (ROS). However, bacterial growth inhibition was also observed under dark conditions and different water quality parameters, indicating that undetermined mechanisms additional to photocatalytic ROS production were responsible for toxicity. The results also indicated that nano-TiO2 particles in the absence and the presence of photoactivation induced lipid peroxidation and cellular respiration disruption.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
Agua, Nuclease-Free Water, for Molecular Biology
Sigma-Aldrich
Agua, sterile-filtered, BioReagent, suitable for cell culture
Sigma-Aldrich
Agua, Deionized
Sigma-Aldrich
Titanium(IV) chloride, ReagentPlus®, 99.9% trace metals basis
Sigma-Aldrich
Agua, for embryo transfer, sterile-filtered, BioXtra, suitable for mouse embryo cell culture
Sigma-Aldrich
Agua, for molecular biology, sterile filtered
Sigma-Aldrich
Titanium(IV) chloride solution, 1.0 M in methylene chloride
Sigma-Aldrich
4,4′-Diaminodiphenylmethane, ≥97.0% (GC)
Sigma-Aldrich
Agua, BioPerformance Certified
Sigma-Aldrich
Agua, ACS reagent
Sigma-Aldrich
Titanium(IV) chloride solution, 0.09 M in 20% HCl
Sigma-Aldrich
Titanium(IV) chloride, ≥99.995% trace metals basis
Sigma-Aldrich
Agua, for cell biology, sterile ultrafiltered
Sigma-Aldrich
Agua, PCR Reagent
Sigma-Aldrich
Disodium succinate, ≥98%
Sigma-Aldrich
Water, deuterium-depleted, ≤1 ppm (Deuterium oxide)
Sigma-Aldrich
Titanium tetrachloride, packaged for use in deposition systems
Sigma-Aldrich
Mechlorethamine hydrochloride, 98%
Sigma-Aldrich
Agua, endotoxin, free