Saltar al contenido
Merck

Quantification of remifentanil and propofol in human plasma: a LC-MS/MS assay validated according to the EMA guideline.

Bioanalysis (2015-08-01)
Jean Claude Alvarez, Emuri Abe, Isabelle Etting, Morgan Le Guen, Philippe Devillier, Stanislas Grassin-Delyle
RESUMEN

Remifentanil and propofol are often used in combination for general anesthesia. We developed a method using LC-MS for their simultaneous measurement in human plasma. Methodology & results: After addition of remifentanil-(13)C6 and propofol-d18 (IS), 500 µl of plasma were extracted with ethylacetate/hexane. Analysis conditions included gradient elution (water/acetonitrile), electrospray ionization and detection with a triple quadripole mass spectrometer. Remifentanil and propofol were ionized in the positive and negative mode, respectively. The method was validated according to the European Medicines Agency guideline for the validation of bioanalytical methods, then successfully applied to clinical samples from three patients who had undergone liver transplantation. This method is suitable for the simultaneous quantification of remifentanil and propofol in clinical studies.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
Bicarbonato de sodio, powder, BioReagent, for molecular biology, suitable for cell culture, suitable for insect cell culture
Sigma-Aldrich
Acetonitrilo, anhydrous, 99.8%
Sigma-Aldrich
Metanol, anhydrous, 99.8%
Sigma-Aldrich
Ácido cítrico, ACS reagent, ≥99.5%
Sigma-Aldrich
Metanol, JIS special grade, ≥99.8%
Sigma-Aldrich
Acetato de etilo, anhydrous, 99.8%
Sigma-Aldrich
Ácido cítrico, 99%
Sigma-Aldrich
Ácido cítrico, ≥99.5%, FCC, FG
Sigma-Aldrich
Hexano, anhydrous, 95%
Sigma-Aldrich
Ácido cítrico, anhydrous, suitable for cell culture, suitable for plant cell culture
Sigma-Aldrich
Ácido cítrico, BioUltra, anhydrous, ≥99.5% (T)
Sigma-Aldrich
Metanol, SAJ first grade, ≥99.5%
Sigma-Aldrich
Ácido fórmico, ≥95%, FCC, FG
Sigma-Aldrich
Propionic acid, ACS reagent, ≥99.5%
Sigma-Aldrich
Acetato de etilo, ≥99%, FCC, FG
Sigma-Aldrich
Acetato de etilo
Sigma-Aldrich
Bicarbonato de sodio, BioXtra, 99.5-100.5%
Sigma-Aldrich
Acetonitrilo, HPLC Plus, ≥99.9%, poly-coated bottles
Sigma-Aldrich
Hexano, JIS special grade, ≥96.0%
Sigma-Aldrich
Propionic acid, ≥99.5%, FCC, FG
Sigma-Aldrich
Acetonitrilo solution, contains 0.1 % (v/v) formic acid, suitable for HPLC
Sigma-Aldrich
Acetonitrilo solution, contains 0.1 % (v/v) trifluoroacetic acid, suitable for HPLC
Sigma-Aldrich
2,6-Diisopropylphenol, 97%
Sigma-Aldrich
Acetonitrilo, electronic grade, 99.999% trace metals basis
Sigma-Aldrich
Acetato de etilo, natural, ≥99%, FCC, FG
Sigma-Aldrich
2-Hydroxybutyric acid sodium salt, 97%
Sigma-Aldrich
Metanol, SAJ special grade
Sigma-Aldrich
Propionic acid, natural, 99%, FG
Sigma-Aldrich
Metanol, suitable for HPLC
Sigma-Aldrich
2-Ethylaniline, 98%