Saltar al contenido
Merck
  • B cell precursor growth-promoting activity. Purification and characterization of a growth factor active on lymphocyte precursors.

B cell precursor growth-promoting activity. Purification and characterization of a growth factor active on lymphocyte precursors.

The Journal of experimental medicine (1988-03-01)
A E Namen, A E Schmierer, C J March, R W Overell, L S Park, D L Urdal, D Y Mochizuki
RESUMEN

We have used a biological assay system we developed to biochemically purify a previously uncharacterized murine lymphopoietic growth factor designated lymphopoietin 1 (LP-1). This factor is capable of stimulating the proliferation and extended maintenance of precursor cells of the B lineage. A stromal cell line producing LP-1 was established after transfection of primary stromal cultures with a plasmid encoding the transforming genes of SV40. This factor was purified to a single 25-kD species from the culture supernatant of an adherent stromal cell line. This material acts on immature lymphocytes, it binds to specific receptors on cells, and is distinct from previously described hematopoietic factors. LP-1 has been purified some 10(7)-fold with an overall recovery of 35%. The purified protein exhibits a specific activity of approximately 4 X 10(6) U/micrograms of protein and is active at a half-maximal concentration of 10(-13) M.

MATERIALES
Referencia del producto
Marca
Descripción del producto

Sigma-Aldrich
IL-7 human, Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)
Sigma-Aldrich
IL-7 from rat, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture