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  • Efflux pumps, OprD porin, AmpC beta-lactamase, and multiresistance in Pseudomonas aeruginosa isolates from cystic fibrosis patients.

Efflux pumps, OprD porin, AmpC beta-lactamase, and multiresistance in Pseudomonas aeruginosa isolates from cystic fibrosis patients.

Antimicrobial agents and chemotherapy (2010-03-03)
Maria Tomás, Michel Doumith, Marina Warner, Jane F Turton, Alejandro Beceiro, German Bou, David M Livermore, Neil Woodford
RESUMEN

Expression of ampC, oprD, mexA, mexC, mexE, and mexX was studied in 25 Pseudomonas aeruginosa isolates from cystic fibrosis patients, including 14 isolates of the Liverpool epidemic strain. Overexpressed mexA or ampC and reduced oprD were associated with beta-lactam resistance. A specific combination of mexR, nalC, and nalD mutations occurred in 11 Liverpool strain isolates, including 7 with upregulated mexA.

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Carbenicillin, Ready Made Solution, 100 mg/mL in ethanol/water, 0.2 μm filtered
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Ciprofloxacin, ≥98% (HPLC)