ZRB1901
Anti-p-RPS6 (Ser240/244) Antibody, clone 2H10 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Sinónimos:
40S ribosomal protein S6;Phosphoprotein NP33;Small ribosomal subunit protein eS6
About This Item
Productos recomendados
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
2H10, recombinant monoclonal
description
2H10 Clone
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 28.68 kDa
observed mol wt ~32 kDa
purified by
using Protein A
species reactivity
mouse, human
species reactivity (predicted by homology)
porcine, canine, bovine, monkey
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
affinity binding assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG
epitope sequence
C-terminal
Protein ID accession no.
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
phosphorylation (pSer240/pSer244)
Gene Information
human ... RPS6(6194)
General description
Specificity
Immunogen
Application
Evaluated by Western Blotting in lysate from NIH3T3 cells treated with PMA.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected phospho-Ribosomal Protein S6 (RPS6) (Ser240/244) in lysates from overnight serum starved NIH3T3 cells treated with 12-O-Tetradecanoylphorbol-13-acetate (PMA; 400 ng/mL; 20 min).
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected p-RPS6 (Ser240/244) in lysate from overnight serum starved A431cells treated with EGF (50 ng/mL; 20 min).
Peptide Inhibition Assay: Target band detection in lysates from overnight serum starved A431 cells treated with EGF (50 ng/mL; 20 min) was prevented by preblocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected p-RPS6 (Ser240/244) in human colon cancer tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected p-RPS6 (Ser240/244) in overnight serum starved A431 cells treated with EGF (50 ng/mL; 15 min.)
Affinity Binding Assay: A representative lot of this antibody bound phospho-Ribosomal Protein S6-(Ser240/244) peptide with a KD of 1.8 x 10-7 in an affinity binding assay.
Flow Cytometry Analysis: 0.1 μg from a representative lot detected p-RPS6 (Ser240/244) in one million A431 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Physical form
Reconstitution
Storage and Stability
Legal Information
Disclaimer
¿No encuentra el producto adecuado?
Pruebe nuestro Herramienta de selección de productos.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico