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Key Documents

SRP6147

Sigma-Aldrich

Ubiquitin Activating Enzyme E1 human

≥95% (SDS-PAGE)

Sinónimos:

AMCX1, POC20, UBA1, UBE1, Ubiquitin E1

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

assay

≥95% (SDS-PAGE)

form

liquid

potency

>3 nM

mol wt

118

packaging

pkg of 50 μg

NCBI accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... UBA1(7317)

General description

Research area: Cell Signaling

Ubiquitin activating enzyme 1 (UBA1) or UBE1, is an ubiquitin-activating enzyme. The gene encoding it is localized on human chromosome Xp11.23.

Application

Ubiquitin Activating Enzyme E1 human has been used in in vitro and in vivo ubiquitination assays.

Biochem/physiol Actions

Ubiquitin activating enzyme 1 (UBA1) has a role in cellular protein homeostasis. It is the first enzyme utilized in the pathway to conjugate ubiquitin onto target substrates through an ubiquitin-protein isopeptide bond. The C-terminal glycine of an ubiquitin (G76) is initially adenylated in an ATP dependent manner activating it so it can be transferred to the active site cysteine of UBE1 forming an ubiquitin-UBE1 thiolester. With the help of the other enzymes in the ubiquitin conjugation pathway (E2/E3 enzymes) the ubiquitin is transferred to a specific lysine on a target substrate. The protein has been associated with spinal muscular atrophy (SMA) and Huntington′s disease (HD).

Physical form

In 50 mM Tris (pH 8.0), 100 mM NaCl and 10% glycerol.

Preparation Note

Centrifuge the vial prior to opening.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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