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SEQR

Sigma-Aldrich

SeqPlex RNA Amplification Kit

For use with high throughput sequencing technologies

Sinónimos:

WGA kit, Whole transcriptome amplification

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About This Item

UNSPSC Code:
41121800
NACRES:
NA.55

technique(s)

whole genome amplification: suitable

shipped in

wet ice

storage temp.

−20°C

Categorías relacionadas

General description

The SeqPlex™ RNA Amplification Kit for whole transcriptome amplification (WTA) is designed to facilitate next-generation sequencing (NGS) from small quantities or from degraded/highly fragmented RNA (e.g. RNA from formalin-fixed paraffin-embedded (FFPE) tissue samples). The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented RNA samples for input into a NGS workflow. It also facilitates the amplification of non-polyA tailed RNA isolated from tissue, cultured cells, formalin-fixed samples, or serum while maintaining patterns of differential expression found in the unamplified sample.

Application

SeqPlex™ RNA Amplification Kit has been used to amplify poly(A)-selected RNA.

Features and Benefits

  • Low quantities of total RNA random priming technology amplifies fragmented or intact RNA from all sources including FFPE and RIP.
  • Semi-degenerate library primer design for more complete transcriptome coverage and efficient priming.
  • No need to fragment DNA before sequencing.
  • Amplifies ds-cDNA in 8 hours or less.
  • Compatible with all next generation sequencing platforms except Pacific Bioscience.

Other Notes

SEQR-500RXN is manufactured on-demand. Contact us for more information.

Legal Information

SeqPlex is a trademark of Sigma-Aldrich Co. LLC

related product

Referencia del producto
Descripción
Precios

T3038

Trizma® hydrochloride solution, 1 M, BioReagent, for molecular biology

S9430

SYBR® Green I nucleic acid gel stain, 10,000 × in DMSO

W4502

Agua, Nuclease-Free Water, for Molecular Biology

AMPD1

DNase I, Amplification Grade

S4438

SYBR® Green JumpStart™ Taq ReadyMix™, for quantitative PCR, MgCI2 in buffer

NA1020

Kit de limpieza para PCR GenElute™, sufficient for 70 purifications

WTA1

TransPlex® Whole Transcriptome Amplification Kit, DNA polymerase separate.

WTA2

TransPlex® Complete Whole Transcriptom Amplification Kit, DNA polymerase included, Complete Kit with optimized enzyme to amplify total RNA in <4 hours, no 3′ bias

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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DNA Sequencing Research Group (DSRG): Evaluation of RNA Amplification Kits at Subnanogram Input Amounts of Total RNA for RNA-Seq
Nicolet C, et al.
Journal of biomolecular techniques : JBT, 24, S70-S70 (2013)
Meritxell Jodar et al.
Human reproduction update, 19(6), 604-624 (2013-07-17)
BACKGROUND Spermatozoa are highly differentiated, transcriptionally inert cells characterized by a compact nucleus with minimal cytoplasm. Nevertheless they contain a suite of unique RNAs that are delivered to oocyte upon fertilization. They are likely integrated as part of many different
Isaac Engel et al.
Nature immunology, 17(6), 728-739 (2016-04-19)
Natural killer T cells (NKT cells) have stimulatory or inhibitory effects on the immune response that can be attributed in part to the existence of functional subsets of NKT cells. These subsets have been characterized only on the basis of
Grégory Seumois et al.
Nature immunology, 15(8), 777-788 (2014-07-07)
A characteristic feature of asthma is the aberrant accumulation, differentiation or function of memory CD4(+) T cells that produce type 2 cytokines (TH2 cells). By mapping genome-wide histone modification profiles for subsets of T cells isolated from peripheral blood of
Kathryn D Tuttle et al.
Nature communications, 9(1), 2650-2650 (2018-07-10)
During development in the thymus, invariant natural killer T (iNKT) cells commit to one of three major functionally different subsets, iNKT1, iNKT2, and iNKT17. Here, we show that T cell antigen receptor (TCR) signal strength governs the development of iNKT
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Protocolos

SEQR - SeqPlex RNA Amplification Kit Protocol

The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.

SEQR - SeqPlex RNA Amplification Kit Protocol

The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.

SEQR - SeqPlex RNA Amplification Kit Protocol

The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.

SEQR - SeqPlex RNA Amplification Kit Protocol

The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.

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