CHA00011
EUR-2019-nCoV Assays for detection of SARS-CoV-2 RNA
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About This Item
UNSPSC Code:
41105500
NACRES:
NA.51
Productos recomendados
form
solution (in 10 mM Tris, 1 mM EDTA)
packaging
pkg of 2 vials
pH range
7.5-8.5
shipped in
ambient
storage temp.
−20°C
General description
EUR-2019-nCoV Assays are for qPCR (5′ nuclease assay) detection of SARS-CoV-2.
The product is primers and probes, the sequences of which were designed and made publicly available by Charité Institute of Virology – Universitätsmedizin Berlin.
For additional details, please visit SigmaAldrich.com/ncov.
The product is primers and probes, the sequences of which were designed and made publicly available by Charité Institute of Virology – Universitätsmedizin Berlin.
For additional details, please visit SigmaAldrich.com/ncov.
Features and Benefits
The forward and reverse primers in each tube come with the following features:
The Dual-Labeled Probe in each tube comes with the following features:
- DNA bases
- No modifications
- Desalt purified
- 8 nmol each E_Sarbeco & 10 nmol each Modified RdRp
The Dual-Labeled Probe in each tube comes with the following features:
- DNA bases
- 6-FAM™ reporter
- BXQ (Block All Quencher—SigmaAldrich.com/bxq)
- HPLC purified
- 4 nmol E_Sarbeco & 5 nmol Modified RdRp
Components
The product includes 2 individual tubes of 20X primer and probe pools for quantification of SARS-CoV-2 genes. Assays included are E_Sarbeco and Modified RdRp (formerly Discrimatory) genes.
The forward primer, reverse primer, and probe are at final concentrations of 8, 8, and 4 μM (E_Sarbeco); and, 10, 10, and 5 μM (Modified RdRp), respectively.
E_Sarbeco Assay:
Forward Primer ACAGGTACGTTAATAGTTAATAGCGT
Reverse Primer ATATTGCAGCAGTACGCACACA
Dual-Labeled Probe ACACTAGCCATCCTTACTGCGCTTCG
Modified RdRp Assay:
Forward Primer AAATGGTCATGTGTGGCGGT
Reverse Primer GTTAAAAACACTATTAGCATAAGCAGTTGT
Dual-Labeled Probe CAGGTGGAACCTCATCAGGAGATGC
The forward primer, reverse primer, and probe are at final concentrations of 8, 8, and 4 μM (E_Sarbeco); and, 10, 10, and 5 μM (Modified RdRp), respectively.
E_Sarbeco Assay:
Forward Primer ACAGGTACGTTAATAGTTAATAGCGT
Reverse Primer ATATTGCAGCAGTACGCACACA
Dual-Labeled Probe ACACTAGCCATCCTTACTGCGCTTCG
Modified RdRp Assay:
Forward Primer AAATGGTCATGTGTGGCGGT
Reverse Primer GTTAAAAACACTATTAGCATAAGCAGTTGT
Dual-Labeled Probe CAGGTGGAACCTCATCAGGAGATGC
Legal Information
FAM is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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