86826

5-Carboxyfluorescein

BioReagent, suitable for fluorescence, ≥95% (HPLC)

• Sinónimos: 5-FAM
• Fórmula empírica (notación de Hill): C₂₁H₁₂O₇
• Número de CAS: 76823-03-5
• Peso molecular: 376.32
• Beilstein/REAXYS Number: 57037
• MDL number: MFCD00036874
• UNSPSC Code: 12171500
• PubChem Substance ID: 57653195
• NACRES: NA.32

Propiedades

• product line: BioReagent
• assay: ≥95% (HPLC)
• form: powder
• solubility: DMF: soluble; DMSO: soluble; H₂O: soluble
• fluorescence: λ_ex 492 nm; λ_em 517 nm in 0.1 M Tris pH 8.0
• suitability: suitable for fluorescence
• SMILES string: OC(=O)c1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35
• InChI: 1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)
• Inchi Key: NJYVEMPWNAYQQN-UHFFFAOYSA-N

Descripción

General description

5-Carboxyfluorescein, also known as 5-FAM, is a green fluorescent product. It is derived from cleavage of 5-Carboxyfluorescein diacetate by intracellular esterases. In viable cells, the fluorescent molecule is impermeable to the cellular membrane.

Application

5-Carboxyfluorescein has been used as a payload to study leakage phenomena on sesame (Sesamum indicum L.) and coconut (Cocos nucifera L.) liposomes.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Derivative of fluorescein giving stable derivative upon conjugation with biopolymers

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)