B6529

Brilliant Blue R Staining Solution

ethanol solution

• Sinónimos: Brilliant Blue R, Acid Blue 83, Brilliant indocyanin 6B, Coomassie Brilliant Blue R
• Fórmula empírica (notación de Hill): C₄₅H₄₄N₃NaO₇S₂
• Número de CAS: 6104-59-2
• Peso molecular: 825.97
• Colour Index Number: 42660
• Beilstein/REAXYS Number: 5718025
• MDL number: MFCD00041762
• UNSPSC Code: 12171500
• PubChem Substance ID: 24891914
• NACRES: NA.47

Propiedades

• product name: Brilliant Blue R Staining Solution, suitable for (for immunoelectrophoresis protein staining)
• form: liquid
• Quality Level: 200
• color: dark blue
• suitability: suitable for (for immunoelectrophoresis protein staining)
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=C/C(C=C5)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1
• InChI: 1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53,54);/q;+1/p-1
• InChI key: NKLPQNGYXWVELD-UHFFFAOYSA-M

Descripción

Application

Brilliant Blue R staining solution is especially designed for use in staining protein in agarose gels following immunoelectrophoresis and Ouchterlony gels. The stain contains ethanol and acetic acid so gels do not require fixing prior to staining. The immunoelectrophoresis or Ouchterlony gel is first washed with water and saline to remove non-precipitated proteins and then dried. The gel is then immersed in staining solution for 30 min and destained with 10% acetic acid.

For detection of protein bands following electrophoresis.

Components

0.5% (w/v) Brilliant Blue R, 45% (v/v) ethanol and 10% (v/v) acetic acid.

Analysis Note

Tested for suitability on agarose gels following immunoelectrophoresis.

Legal Information

Información de seguridad

• pictograms: GHS02,GHS07
• signalword: Warning
• hcodes: H226,H315,H319
• pcodes: P210 - P233 - P240 - P241 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 3 - Skin Irrit. 2
• Storage Class: 3 - Flammable liquids
• wgk_germany: WGK 2
• flash_point_f: 81.0 °F - closed cup
• flash_point_c: 27.2 °C - closed cup
• ppe: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter