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AM80

Sigma-Aldrich

Anti-PAR (Ab-1) Mouse mAb (10H)

liquid, ≥95% (SDS-PAGE), clone 10H

Sinónimos:

Anti-Poly (ADP-Ribose)

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

10H, monoclonal

assay

≥95% (SDS-PAGE)

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human, Drosophila, rat, mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG3

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Protein A purified mouse monoclonal antibody. Recognizes poly-ADP-ribose.
Recognizes poly(ADP)-ribose (PAR) in etopside-treated HL-60 cells.
This Anti-PAR (Ab-1) Mouse mAb (10H) is validated for use in Immunoblotting, Immunocytochemistry for the detection of PAR (Ab-1).

Immunogen

Bovine
purified poly(ADP-ribose)

Application

Immunoblotting (2.5 µg/ml)

Immunocytochemistry (5-20 µg/ml)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 100 mM NaCl, 50 mM HEPES, 1% BSA, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Negative Control
Normal mouse IgG
Positive Control
Etoposide-treated HL-60 cells

Other Notes

Kawamitsu, H., et al. 1984. Biochemistry23, 3771.
PAR can be induced in HL-60 cells by treatment with 67 µM etoposide for 3.5 h. Antibody should be titrated for optimal results in an individual system.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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