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  • A new validated HPLC method for the determination of quercetin: Application to study pharmacokinetics in rats.

A new validated HPLC method for the determination of quercetin: Application to study pharmacokinetics in rats.

Biomedical chromatography : BMC (2016-08-25)
Khaled S Abdelkawy, Mark E Balyshev, Fawzy Elbarbry
PMID27555122
ABSTRACT

A simple, accurate, and reproducible high-performance liquid chromatography (HPLC) method has been developed and validated for the quantification of quercetin (QR) in rat plasma. The method involves a simple protein precipitation procedure to extract both QR and thymoquinone (TQ), the internal standard. The chromatographic analysis was achieved on a Shimadzu LC 20ā€‰A HPLC system equipped with a Supelcosil LC-18ā€‰T C

MATERIALS
Product Number
Brand
Product Description
59136-U
Supelco
SUPELCOSILā„¢ LC-18-T HPLC Column, 5 Ī¼m particle size, L Ɨ I.D. 15 cm Ɨ 4.6 mm
58970-U
Supelco
SUPELCOSILā„¢ LC-18-T HPLC Column, 3 Ī¼m particle size, L Ɨ I.D. 15 cm Ɨ 4.6 mm
59621
Supelco
SUPELCOSILā„¢ LC-18-T Supelguard Cartridge, 5 Ī¼m particle size, L Ɨ I.D. 2 cm Ɨ 4 mm
59132
Supelco
SUPELCOSILā„¢ LC-NH2-NP HPLC Column, 5 Ī¼m particle size, L Ɨ I.D. 25 cm Ɨ 4.6 mm
58970C30
Supelco
SUPELCOSILā„¢ LC-18-T HPLC Column, 3 Ī¼m particle size, L Ɨ I.D. 15 cm Ɨ 3 mm
58928-U
Supelco
SUPELCOSILā„¢ LC-18-S HPLC Column, 5 Ī¼m particle size, L Ɨ I.D. 25 cm Ɨ 4.6 mm
59564C30
Supelco
SUPELCOSILā„¢ LC-18 Supelguard Cartridge, 5 Ī¼m particle size, L Ɨ I.D. 2 cm Ɨ 3 mm
59516
Supelco
SUPELCOSILā„¢ LC-NH2-NP Supelguard Cartridge, 5 Ī¼m particle size, L Ɨ I.D. 2 cm Ɨ 4 mm
59621C30
Supelco
SUPELCOSILā„¢ LC-18-T Supelguard Cartridge, 5 Ī¼m particle size, L Ɨ I.D. 2 cm Ɨ 3 mm
59613
Supelco
SUPELCOSILā„¢ LC-18 Supelguard Cartridge, 5 Ī¼m particle size, L Ɨ I.D. 2 cm Ɨ 2.1 mm
59554
Supelco
SUPELCOSILā„¢ LC-18 Supelguard Cartridge, 5 Ī¼m particle size, L Ɨ I.D. 2 cm Ɨ 4 mm
59620
Supelco
SUPELCOSILā„¢ LC-18-T Supelguard Cartridge, 5 Ī¼m particle size, L Ɨ I.D. 2 cm Ɨ 4 mm
57939
Supelco
SUPELCOSILā„¢ LC-18-S HPLC Column, 5 Ī¼m particle size, L Ɨ I.D. 25 cm Ɨ 2.1 mm
58971
Supelco
SUPELCOSILā„¢ LC-18-T HPLC Column, 5 Ī¼m particle size, L Ɨ I.D. 25 cm Ɨ 4.6 mm
58931
Supelco
SUPELCOSILā„¢ LC-18-S HPLC Column, 5 Ī¼m particle size, L Ɨ I.D. 15 cm Ɨ 4.6 mm
59564
Supelco
SUPELCOSILā„¢ LC-18 Supelguard Cartridge, 5 Ī¼m particle size, L Ɨ I.D. 2 cm Ɨ 4 mm