Skip to Content

Space constraints govern fate of hematopoietic stem and progenitor cells in vitro.

Space constraints govern fate of hematopoietic stem and progenitor cells in vitro.

Biomaterials (2015-04-22)

Eike Müller, Tatyana Grinenko, Tilo Pompe, Claudia Waskow, Carsten Werner

ABSTRACT

Deciphering exogenous cues that determine stem cell fate decisions is a persisting challenge of cell biology and bioengineering. In an effort to unravel the role of spatial constraints in the cell-instructive characteristics of bone marrow microenvironments, murine hematopoietic stem and progenitor cells (HSPC) were exposed to fibronectin-coated microcavities in vitro. Microcavity sizes were chosen to allow for the inclusion of either individual or multiple cells. Repopulation experiments using lethally irradiated mice showed that the maintenance of functional HSPC in culture critically depends on cavity dimensions. Short-term repopulating hematopoietic stem cells (ST-HSC) were found to be best supported within single-cell sized compartments while long-term repopulating HSC (LT-HSC) were maintained within both cavity sizes. In sum, the reported data reveal spatial restriction to be a simple but powerful means for directing HSPC fate ex vivo.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Ethanol, ≥99.5%

Sigma-Aldrich

2-Propanol, SAJ first grade, ≥99.0%

Sigma-Aldrich

2-Propanol, JIS special grade, ≥99.5%

Sigma-Aldrich

Ethanol, JIS 300, ≥99.5%, for residue analysis

Sigma-Aldrich

Ethanol, ≥99.5%, SAJ super special grade

Sigma-Aldrich

Ethanol, JIS 1000, ≥99.5%, for residue analysis

Sigma-Aldrich

Ethanol, ≥99.5%, suitable for absorption spectrum analysis

Sigma-Aldrich

Ethanol, ≥99.5%, suitable for fluorescence

Sigma-Aldrich

2-Propanol, BioUltra, for molecular biology, ≥99.5% (GC)

Sigma-Aldrich

2-Propanol, anhydrous, 99.5%

Sigma-Aldrich

2-Propanol, 99.5%, HPLC grade

Sigma-Aldrich

Ethyl alcohol, Pure, 190 proof, ACS spectrophotometric grade, 95.0%

Sigma-Aldrich

Ethyl alcohol, Pure, 190 proof, meets USP testing specifications

Sigma-Aldrich

2-Ethyl-2-phenylmalonamide monohydrate, 99%

Supelco

Ethanol standards 10% (v/v), 10 % (v/v) in H₂O, analytical standard

Sigma-Aldrich

2-Propanol, HPLC Plus, for HPLC, GC, and residue analysis, 99.9%, poly coated bottles

Sigma-Aldrich

2-Propanol, for molecular biology, BioReagent, ≥99.5%

Sigma-Aldrich

2-Propanol, electronic grade, 99.999% trace metals basis

Sigma-Aldrich

(3-Aminopropyl)triethoxysilane, 99%

Sigma-Aldrich

(3-Aminopropyl)triethoxysilane, packaged for use in deposition systems, ≥98%

Sigma-Aldrich

(3-Aminopropyl)triethoxysilane, ≥98.0%

Sigma-Aldrich

Hydrogen chloride, ReagentPlus^®, ≥99%

Sigma-Aldrich

Ethyl alcohol, Pure, 200 proof, anhydrous, ≥99.5%

Sigma-Aldrich

Ethanol, ≥99.5%, suitable for HPLC

Sigma-Aldrich

DAPI, for nucleic acid staining

Sigma-Aldrich

2-Propanol, suitable for HPLC

Sigma-Aldrich

(3-Aminopropyl)triethoxysilane, ≥98%

Sigma-Aldrich

2-Propanol, ACS reagent, ≥99.5%

Sigma-Aldrich

Heptane, SAJ first grade, ≥98.0%

Sigma-Aldrich

Heptane, anhydrous, 99%