Generation of iPSC line HEL24.3 from human neonatal foreskin fibroblasts.

Generation of iPSC line HEL24.3 from human neonatal foreskin fibroblasts.

Stem cell research (2015-06-22)

Ras Trokovic, Jere Weltner, Timo Otonkoski

ABSTRACT

Human iPSC line HEL24.3 was generated from healthy human foreskin fibroblasts using non-integrative reprogramming method. Reprogramming factors Oct3/4, Sox2, Klf4, and cMyc were delivered using Sendai viruses.

MATERIALS

Product Number

Brand

Product Description

19-1335

Sigma-Aldrich

2-Mercaptoethanol, ≥99.0%

19-1330

Sigma-Aldrich

2-Mercaptoethanol, SAJ special grade, ≥99.0%

63689

Sigma-Aldrich

2-Mercaptoethanol, BioUltra, for molecular biology, ≥99.0% (GC)

M6250

Sigma-Aldrich

2-Mercaptoethanol, ≥99.0%

M3148

Sigma-Aldrich

2-Mercaptoethanol, for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

V900464

Sigma-Aldrich

Sodium butyrate, Vetec^™, reagent grade, 99%

09-1320

Sigma-Aldrich

Ethylenediaminetetraacetic acid, SAJ special grade, ≥99.0%

431788

Sigma-Aldrich

Ethylenediaminetetraacetic acid, 99.995% trace metals basis

303410

Sigma-Aldrich

Sodium butyrate, 98%

D9542

Sigma-Aldrich

DAPI, for nucleic acid staining

E6758

Sigma-Aldrich

Ethylenediaminetetraacetic acid, anhydrous, crystalline, BioReagent, suitable for cell culture

Sigma-Aldrich

Ethylenediaminetetraacetic acid, purified grade, ≥98.5%, powder

EDS

Sigma-Aldrich

Ethylenediaminetetraacetic acid, anhydrous, BioUltra, ≥99% (titration)

B5887

Sigma-Aldrich

Sodium butyrate, ≥98.5% (GC)

E9884

Sigma-Aldrich

Ethylenediaminetetraacetic acid, ACS reagent, 99.4-100.6%, powder

E8008

Sigma-Aldrich

Ethylenediaminetetraacetic acid solution, 0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture