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C6974

Sigma-Aldrich

Anti-CaM Kinase IIα (CaMKIIα) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.44

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 50 kDa

species reactivity

human, chicken, rat, mouse

packaging

antibody small pack of 25 μL

technique(s)

microarray: suitable
western blot: 1:5,000 using synaptosomal fraction of rat brain

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CAMK2A(815)
mouse ... Camk2a(12322)
rat ... Camk2a(25400)

General description

Ca2+/Calmodulin dependent protein kinase II (CaMKII) belongs to the family of Ser/Thr protein kinases including CaMKI and CaMKIV. CaM kinase II is one of the most abundant protein kinases in the mammalian brain, with the highest expression in neurons of the hippocampus and the cerebral cortex. CaMKII consists of a family of four related isoforms CaMK IIα, CaMK IIβ, CaMK IIγ and CaMK IIδ. The CaMK II α and β isoforms are predominantly expressed in the brain, localized mainly in the cytosol and postsynaptic densities (PSDs), whereas the CaMKII γ and δ isoforms are expressed in all tissues. CaMKII contains a catalytic and regulatory domain. The regulatory domain consists of an autoinhibitory and calmodulin binding site.

Specificity

The antibody does not cross-react with CaMKIIβ, γ and δ isoforms (60 kDa). Anti-CaM Kinase IIα specifically binds to CaM Kinase IIα (50kDa) in human, mouse, rat and chicken. CaM Kinase IIα immunizing peptide specifically inhibits the staining of CaM Kinase IIα in immunoblotting.

Immunogen

Peptide corresponding to amino acids 461-478 of rat CaM Kinase Iiα, conjugated to KLH. This sequence is identical in human, mouse and chicken CaMKIIα has limited homology (50-60%) with CaM Kinase IIβ, γ and δ subunits.

Application

Anti-CaM Kinase IIα (CaMKIIα) antibody has been used:
  • in immunolabelling
  • in western blotting
  • in immunoprecipitation
  • in immunoblotting

Biochem/physiol Actions

CaMKII is involved in many cellular functions in response to Ca2+ signaling, including synthesis and secretion of neurotransmitters, axonal transport, long term potentiation (LTP) and spatial learning, receptor function and regulation of gene expression. CaMKII plays a critical role in LTP, a cellular model of learning and memory. In the CNS, postsynaptic Ca2+ influx triggers rapid autophosphorylation and stable activation of CaMKII in a Ca2+/calmodulin dependent manner at a threonine residue in the autoinhibitory domain. Autophosphorylation of CaMKIIα at Thr286 has been shown to be required for LTP and learning.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Structure of the autoinhibited kinase domain of CaMKII and SAXS analysis of the holoenzyme
Rosenberg O S, et al.
Cell, 123(5), 849-860 (2005)
CaMKII beta functions as an F-actin targeting module that localizes CaMKII alpha beta heterooligomers to dendritic spines
Shen K, et al.
Neuron, 21(3), 593-606 (1998)
Sophie Riedemann et al.
The Journal of comparative neurology, 527(14), 2215-2232 (2019-03-09)
Transgenic animals have become a widely used model to identify and study specific cell types in whole organs. Promotor-driven reporter gene labeling of the cells under investigation has promoted experimental efficacy to a large degree. However, rigorous assessment of transgene
GABAergic mechanisms regulated by miR-33 encode state-dependent fear
JovasevicV, et al.
Nature Neuroscience, 18(9), 1265-1265 (2015)
Structure-function of the multifunctional Ca2+/calmodulin-dependent protein kinase II
Hudmon, A and Schulman H
The Biochemical Journal, 364(3), 593-611 (2002)

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