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Key Documents

SCT276

Sigma-Aldrich

BioTracker™ Green pH-Independent Autophagy Live Cell Probe

Human

Synonym(s):

autophagosome live cell probe, green AP live cell probe, green autophagosome probe, green autophagy probe

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About This Item

UNSPSC Code:
41106514
NACRES:
NA.47

product name

BioTracker™ Green pH-Independent Autophagy Live Cell Probe,

biological source

human

Quality Level

100

packaging

vial of 5 nmol

manufacturer/tradename

Millipore

technique(s)

cell culture | stem cell: suitable
flow cytometry: suitable

shipped in

2-8°C

storage temp.

2-8°C

General description

SCT276 is a small fluorescent molecule that detects autophagosomes and autolysosomes by a likely mechanism of incorporation into the autophagosome double membrane during its formation, whereupon it emits fluorescence under hydrophobic conditions. This probe has been noted to frequently colocalize with LC3. SPECTRAL CHARACTERISTICS: Excitation λ: 450 nmEmission λ: 535 nm

Features and Benefits

Fluorescence microscopyFlow cytometry

Target description

Autophagy is an intracellular degradation system, where dysfunctional proteins and organelles are degraded. In this process, dysfunctional proteins aggregate and are enclosed by a double membrane to form an autophagosome.

Storage and Stability

Store at -20 °C, protected from light.

Other Notes

SCT276 detects autophagosomes and autolysosomes by the likely mechanism of incorporation into the autophagosome during double membrane formation, and emits green fluorescence under hydrophobic conditions. SCT276 is cell permeable, does not require transfection, and enables live cell imaging with fluorescence microscopy and quantitative assay by flow cytometry.Add 50 uL DMSO to tube of SCT276 (5 nmol) and pipet to dissolve to make a 0.1 mM stock solution. Dilute with culture medium to 0.1 - 0.5 uM to make working solution.

Legal Information

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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