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Key Documents

09-432

Sigma-Aldrich

Anti-phospho-IRS1 (Tyr608) mouse/ (Tyr612) human Antibody

Upstate®, from rabbit

Synonym(s):

HIRS-1, Insulin Receptor Substrate 1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

phosphorylation (pTyr608 )

Gene Information

human ... IRS1(3667)

General description

Insulin receptor substrate (IRS) molecules are key mediators in insulin signaling and play a central role in maintaining basic cellular functions such as growth, survival, and metabolism. They act as docking proteins between the insulin receptor and a complex network of intracellular signaling molecules containing Src homology 2 (SH2) domains. Four members (IRS-1, IRS-2, IRS-3, IRS-4) of this family have been identified that differ as to tissue distribution, subcellular localization, developmental expression, binding to the insulin receptor, and interaction with SH2 domain-containing proteins. Results from targeted disruption of the IRS genes in mice have provided important clues to the functional differences among these related molecules, suggesting they play different and specific roles in vivo. The available data are consistent with the notion that IRS-1 and IRS-2 are not functionally interchangeable in tissues that are responsible for glucose production (liver), glucose uptake (skeletal muscle and adipose tissue), and insulin production (pancreatic β cells). In fact, IRS-1 appears to have its major role in skeletal muscle whereas IRS-2 appears to regulate hepatic insulin action as well as pancreatic β cell development and survival. By contrast, IRS-3 and IRS-4 genes appear to play a redundant role in the IRS signaling system. Defects in muscle IRS-1 expression and function have been reported in insulin-resistant states such as obesity and type 2 diabetes.

Specificity

Predicted to cross react with mouse and rat based on 100% sequence homology.
Recognizes phospho-IRS1 (Tyr608) mouse/ (Tyr612) human

Immunogen

peptide corresponding to amino acids encompassing and including phosphorylated Tyr608 in mouse and Tyr612 in human of IRS1

Application

Anti-phospho-IRS1 (Tyr608) mouse/ (Tyr612) human Antibody detects level of phospho-IRS1 (Tyr608) mouse/ (Tyr612) human & has been published & validated for use in WB.
Research Category
Signaling
Research Sub Category
Insulin/Energy Signaling

Quality

Evaluated by Western Blotting in Insulin treated HEK293 cell lysate.

Western Blotting Analysis: 1 mg/mL of this antibody detected phospho-IRS1 (Tyr608) mouse/ (Tyr612) in 10 µg of lysate from Insulin treated HEK293 cells.cells

Target description

~160 kDa observed. 131.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity purified
Affinity purified rabbit polyclonal IgG in storage buffer containing PBS with 0.05% sodium azide in 50% glycerol.

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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An organic matrix-mediated processing methodology to fabricate hydroxyapatite based nanostructured biocomposites.
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High-fat diet induces hepatic insulin resistance and impairment of synaptic plasticity.
Liu, Z; Patil, IY; Jiang, T; Sancheti, H; Walsh, JP; Stiles, BL; Yin, F; Cadenas, E
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Reduced phosphorylation of brain insulin receptor substrate and Akt proteins in apolipoprotein-E4 targeted replacement mice.
Ong, QR; Chan, ES; Lim, ML; Cole, GM; Wong, BS
Scientific Reports null
Weikang Cai et al.
Nature communications, 8, 14892-14892 (2017-03-28)
Despite a high degree of homology, insulin receptor (IR) and IGF-1 receptor (IGF1R) mediate distinct cellular and physiological functions. Here, we demonstrate how domain differences between IR and IGF1R contribute to the distinct functions of these receptors using chimeric and
Muscle-specific knock-out of NUAK family SNF1-like kinase 1 (NUAK1) prevents high fat diet-induced glucose intolerance.
Inazuka, F; Sugiyama, N; Tomita, M; Abe, T; Shioi, G; Esumi, H
The Journal of Biological Chemistry null

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