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  • Phosphatidylethanolamine, but not Phosphatidylglycerol-Cardiolipin, Isolated from Two Species of Helicobacter Binds Cholesterol over Cholesteryl Ester.

Phosphatidylethanolamine, but not Phosphatidylglycerol-Cardiolipin, Isolated from Two Species of Helicobacter Binds Cholesterol over Cholesteryl Ester.

Lipids (2015-07-01)
Avarzed Amgalanbaatar, Kouichi Hosoda, Hirofumi Shimomura
ABSTRACT

This study demonstrated that the cells of Helicobacter felis and Helicobacter cinaedi spontaneously absorb cholesterol added to the medium. A recent study by our group has revealed that phosphatidylethanolamine (PtdEtn) of Helicobacter pylori contains myristic acid as the most predominant saturated fatty acid and that the PtdEtn of this bacterium binds cholesterol more selectively than cholesteryl ester. We, therefore, isolated the PtdEtn from the two Helicobacter species to analyze the hydrophobic interaction between cholesterol and its glycerophospholipid. PtdEtn of the Helicobacter bacteria interacted more selectively with cholesterol than with cholesteryl ester, and the degree of the selective binding of cholesterol was higher in the PtdEtn than in the phosphatidylglycerol-cardiolipin of the same bacteria. These results suggest the possibility that the cells of H. felis and H. cinaedi may contain abundant PtdEtn with myristic acid. On this basis, we analyzed the PtdEtn molecular species of the Helicobacter bacteria and demonstrated that the PtdEtn containing myristic acid accounts for more than 35% in the total PtdEtn. These results suggest that the myristoyl PtdEtn takes part in the absorption of cholesterol in H. felis and H. cinaedi.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
SyntheChol® NS0 Supplement, 500 ×, synthetic cholesterol, animal component-free, aqueous solution, sterile-filtered, suitable for cell culture
Sigma-Aldrich
Cholesterol, powder, BioReagent, suitable for cell culture, ≥99%
Sigma-Aldrich
Cholesterol, from sheep wool, ≥92.5% (GC), powder
Sigma-Aldrich
Cholesterol, Sigma Grade, ≥99%
Sigma-Aldrich
Palmitic acid-d31, 98 atom % D, 99% (CP)
SAFC
Cholesterol, from sheep wool, Controlled origin, meets USP/NF testing specifications
Sigma-Aldrich
Palmitic acid-d31, endotoxin tested, 98 atom % D
SAFC
Cholesterol, Plant-Derived, SyntheChol®