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  • Direct biomolecule binding on nonfouling surfaces via newly discovered supramolecular self-assembly of lysozyme under physiological conditions.

Direct biomolecule binding on nonfouling surfaces via newly discovered supramolecular self-assembly of lysozyme under physiological conditions.

Macromolecular bioscience (2012-06-19)
Peng Yang
ABSTRACT

When lysozyme is dissolved in a neutral HEPES buffer solution (pH = 7.4) with 0.001-0.050 M TCEP added, a fast phase transition process occurs and the resulting novel fiber-like hierarchical supramolecular assemblies made by primary spherical-particle aggregation can function as a "superglue" that binds strongly and quickly onto non-fouling coatings. This binding is highly selective towards lysozyme, and excludes synthetic, chemical/physical activation/deactivation (blocking) steps. By using biotinylated lysozyme, such a phase transition quickly creates a perfect biotinylated surface on non-fouling surfaces for avidin binding, showing great potential for the development of low-cost and practical biochips.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
HEPES sodium salt solution, BioReagent, 1M, suitable for cell culture
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HEPES sodium salt, ≥99.0% (titration)
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SAFC
HEPES
SAFC
HEPES sodium salt
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HEPES, BioPerformance Certified, ≥99.5% (titration), suitable for cell culture
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HEPES, BioUltra, for molecular biology, ≥99.5% (T)
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Sigma-Aldrich
HEPES solution, BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered
Sigma-Aldrich
HEPES buffer solution, 1 M in H2O
Sigma-Aldrich
HEPES solution, 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture