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Key Documents

RAB0503

Sigma-Aldrich

Human TSLP ELISA Kit

for serum, plasma, cell culture supernatants and urine

Synonym(s):

TSLP, Thymic stromal lymphopoietin

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type serum
sample type urine
sample type cell culture supernatant(s)
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 3 pg/mL
standard curve range: 3.28-800 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... TSLP(85480)

General description

The Human TSLP (thymic stromal lymphopoietin) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TSLP in serum, plasma, cell culture supernatants and urine.
Thymic stromal lymphopoietin (TSLP) is an interleukin 7 (IL-7)-like cytokine derived from epithelial cells. TSLP aids in the pathogenesis of allergic diseases such as atopic dermatitis, asthma, and chronic obstructive pulmonary disease by activating B-cells, dendritic cells, and progenitor cells.

Immunogen

Recombinant Human TSLP

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
The Human TSLP (thymic stromal lymphopoietin) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TSLP in serum, plasma, cell culture supernatants, and urine.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABELADDELISA 5X Assay/Sample Diluent Buffer D (Item K)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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TSLP: an epithelial cell cytokine that regulates T cell differentiation by conditioning dendritic cell maturation.
Liu, et al.
Annual Review of Immunology, 25, 193-219 (2020)
Phil-Dong Moon et al.
Amino acids, 42(6), 2513-2519 (2011-08-13)
The cytokine thymic stromal lymphopoietin (TSLP) has been implicated in the development and progression of allergic diseases such as atopic dermatitis, asthma, and chronic obstructive pulmonary disease. However, it has not yet been clarified the effect of epigallocatechin-3-O-gallate (EGCG) on
Gail M Gauvreau et al.
The New England journal of medicine, 370(22), 2102-2110 (2014-05-23)
Thymic stromal lymphopoietin (TSLP) is an epithelial-cell-derived cytokine that may be important in initiating allergic inflammation. AMG 157 is a human anti-TSLP monoclonal immunoglobulin G2λ that binds human TSLP and prevents receptor interaction. In this double-blind, placebo-controlled study, we randomly
S Demehri et al.
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 44(6), 851-857 (2014-01-09)
Mouse models of atopic march suggest that systemic, skin-derived thymic stromal lymphopoietin (TSLP) mediates progression from eczema to asthma. We investigated whether circulating TSLP is associated with eczema, allergic sensitization, or recurrent wheezing in young children. A prospective analysis of
G Mócsai et al.
The British journal of dermatology, 170(3), 617-624 (2013-11-21)
Filaggrin (FLG) deficiency is a well-known predisposing factor for the development of atopic dermatitis (AD). Decreased FLG expression can be the result of haploinsufficiency or severe inflammation, which can cause acquired FLG alterations. FLG mutations are related to several clinical
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