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RAB0374

Sigma-Aldrich

Mouse MPO ELISA Kit

for plasma and cell culture supernatant

Synonym(s):

MPO, Myeloperoxidase

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 600 pg/mL
standard curve range: 0.61-150 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Mpo(17523)

Related Categories

General description

The Mouse MPO (Myeloperoxidase) ELISA (Enzyme- Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MPO in plasma and cell culture supernatants.

Immunogen

Recombinant Mouse Myeloperoxidase

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Myeloperoxidase (MPO) has a crucial role to play in the destruction of various microorganisms and foreign cells, such as bacteria, fungi, viruses, red cells, and malignant and nonmalignant nucleated cells. MPO catalyzes the production of number of reactive oxidant species (ROS) that influence tissue damage during inflammation. MPO and its downstream inflammatory pathways function as a potent therapeutic target for prophylaxis of atherosclerotic cardiovascular disease. MPO catalyzes the synthesis of polychlorinated dibenzo(p)dioxins and furans (PCDD/F) from precursors such as chlorophenols in the presence of hydrogen peroxide (H2O2).

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABELADCELISA 1X Assay/Sample Diluent Buffer C (Item L)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Nour Eissa et al.
Scientific reports, 7, 42427-42427 (2017-02-12)
2,4-Dinitrobenzene sulfonic acid (DNBS)-induced colitis is an experimental model that mimics Crohn's disease. Appropriateness of reference genes is crucial for RT-qPCR. This is the first study to determine the stability of reference gene expression (RGE) in mice treated with DNBS.
Clinical manifestation of myeloperoxidase deficiency.
Lanza F, et al.
Journal of Molecular Medicine, 76, 676-681 (1998)
Sebastian Mayer-Rollnik et al.
European journal of anaesthesiology, 37(3), 180-186 (2019-12-05)
Postoperative autologous retransfusion of drainage blood might reduce the transfusion of red blood cell concentrates after major orthopaedic surgery. Our primary objective was to evaluate the effectiveness of a blood collection and retransfusion system. Secondary objectives included safety issues and
Myeloperoxidase-catalyzed formation of PCDD/F from chlorophenols.
Wittsiepe J, et al.
Chemosphere, 40, 963-968 (2000)
Myeloperoxidase and cardiovascular disease.
Nicholls SJ and Hazen SL
Arteriosclerosis, Thrombosis, and Vascular Biology, 25, 1102-1111 (2005)
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