MAK491

Formate Assay Kit

sufficient for 100 colorimetric tests

• Synonym(s): Formate Test Kit
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• Quality Level: 100
• application(s): environmental
• detection method: colorimetric
• relevant disease(s): neurological disorders
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

Formate is the anion derived from formic acid, the simplest carboxylic acid. It is also the metabolic byproduct of formaldehyde metabolism in the body, and the eventual metabolic byproduct of methanol, which is first broken down to formaldehyde. At high levels, formate is neurotoxic to the central nervous system and can cause blindness, coma, and death. Although naturally present in the body at low levels, elevated levels of formate may be used as an indicator of formaldehyde exposure and methanol poisoning.

Features and Benefits

• Linear detection range 0.050 to 5 mM formate.
• Compatible with high-throughput handling systems.

Suitability

The kit is suitable for the determination of formate in biological samples such as urine and serum.

Principle

The Formate Assay Kit is based on the formate dehydrogenase catalyzed oxidation of formate, which generates carbon dioxide and NADH that reduces a formazan (MTT) dye. The intensity of the reduced MTT, measured at 565 nm, is directly proportional to the formate concentration in the sample.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2