AB5972
Anti-Dishevelled-2 Antibody
Chemicon®, from rabbit
Synonym(s):
DVL2, DSH Homolog 2
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
rabbit
Quality Level
antibody form
purified immunoglobulin (Partially purified)
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, mouse
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
western blot: suitable
input
sample type hematopoietic stem cell(s)
sample type neural stem cell(s)
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... DVL2(1856)
mouse ... Dvl2(13543)
General description
DVL2 is a member of multigene family that also contains DVL1 and DVL3. The vertebrate dishevelled proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. DVL2 encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein.
Specificity
Disheveiled-2 protein (dvl-2). By Western blot the antibody shows no cross reactivity with recombinant disheveiled-1 (dvl-1) or disheveiled-3 (dvl-3) protein. Pre-adsorption of the antibody with the immunogen peptide completely abolishes its reactivity by Western blot against disheveiled-2 protein.
Immunogen
Synthetic peptide corresponding to amino acids 600-614 of human and mouse disheveiled-2 protein.
Application
Detect Dishevelled-2 using this Anti-Dishevelled-2 Antibody validated for use in WB, IH.
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Hematopoietic Stem Cells
Neural Stem Cells
Hematopoietic Stem Cells
Neural Stem Cells
Western blot: 1:1,000 using ECL. The antibody reacts with a protein of ~95kDa. Faint reactivity was seen in the dvl-1 and dvl-3 HEK293 cell lysates suggesting there may be endogenous dvl-2 present.
Immunohistochemistry: 1:1,000 using the avidin-biotin system.
Dilute antibody in 50 mM PBS, pH 7.4 containing 1.5% sodium chloride and 1% normal animal serum (same species as the host of the secondary antibody).
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 1:1,000 using the avidin-biotin system.
Dilute antibody in 50 mM PBS, pH 7.4 containing 1.5% sodium chloride and 1% normal animal serum (same species as the host of the secondary antibody).
Optimal working dilutions must be determined by end user.
Target description
80 kDa
Physical form
Format: Purified
Partially purified immunoglobulin. Liquid in PBS, pH 7.4, containing 0.01M sodium azide.
Storage and Stability
Maintain at -20°C in undiluted aliquots (not less than 10 μL) for up to 6 months. Avoid repeated freeze/thaw cycles. The use of high quality antiserum-grade plastic or glass is recommended.
Analysis Note
Control
SK-N-SH (human neuroblastoma) whole cell lysates, NIH 3T3 whole cell lysate, BT-20 (human mammary gland) cell lysate, FP (mouse embryonal carcinoma
testicular teratoma) cell lysate
SK-N-SH (human neuroblastoma) whole cell lysates, NIH 3T3 whole cell lysate, BT-20 (human mammary gland) cell lysate, FP (mouse embryonal carcinoma
testicular teratoma) cell lysate
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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