Skip to Content
Merck
All Photos(1)

Key Documents

03-0136-00

Millipore

SMC® Mouse IL-4 Discovery Immunoassay Kit

Synonym(s):

mouse IL 4 immunoassay kit, mouse IL-4 Single Molecule Counting Kit, mouse IL-4 ultra-sensitive immunoassay, mouse Interleukin 4 immunoassay kit

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84

species reactivity

mouse

Quality Level

manufacturer/tradename

SMC®

assay range

accuracy: 80-120%
(minimum of 10 samples spiked with acceptable recovery)

technique(s)

ELISA: suitable
single molecule counting: suitable

General description

Interleukin-4 (IL-4) is a cytokine that is a key regulator in humoral and adaptive immunity. IL-4 induces differentiation of naive helper T-cells (Th0 cells) to Th2 cells. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. Further, IL-4 controls the production of IgE, expands IL-4 producing T-cell subsets and stabilizes effector cell functions. IL-4 has great therapeutic potential due to its role in the development of allergic inflammatory responses. IL-4 also has shown to have promise in drug targeting for cancer.
The SMC® Mouse IL-4 Discovery Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC® immunoassay system detects analytes using Single Molecule Counting (SMC®) technology.

Application

Research Category
Inflammation & Immunology
Research Sub Category
Cytokines & Cytokine Receptors
The Single Molecule Counting (SMC®) Mouse IL-4 Discovery Immunoassay has unparalleled performance in quantifying IL-4 in mouse serum.
Used to detect/quantify:IL-4

Components

Assay Buffer
Mouse IL-4 Coating Antibody
Assay Plate
10X PBS
Blocking Buffer
Standard Diluent
Mouse IL-4 Detection Antibody
Mouse IL-4 Standard
SMC® Mouse IL-4 Immunoassay Kit Instructions
10X Wash Buffer
Buffer C
Elution Buffer B

Analysis Note

Low-end CV% Range: 0-20%|Low-end CV% Average: 4%

Legal Information

SMC is a registered trademark of Singulex

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Pictograms

Health hazardExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B - Skin Sens. 1

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service