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  • A Comprehensive Roadmap of Murine Spermatogenesis Defined by Single-Cell RNA-Seq.

A Comprehensive Roadmap of Murine Spermatogenesis Defined by Single-Cell RNA-Seq.

Developmental cell (2018-08-28)
Christopher Daniel Green, Qianyi Ma, Gabriel L Manske, Adrienne Niederriter Shami, Xianing Zheng, Simone Marini, Lindsay Moritz, Caleb Sultan, Stephen J Gurczynski, Bethany B Moore, Michelle D Tallquist, Jun Z Li, Saher Sue Hammoud
ABSTRACT

Spermatogenesis requires intricate interactions between the germline and somatic cells. Within a given cross section of a seminiferous tubule, multiple germ and somatic cell types co-occur. This cellular heterogeneity has made it difficult to profile distinct cell types at different stages of development. To address this challenge, we collected single-cell RNA sequencing data from ∼35,000 cells from the adult mouse testis and identified all known germ and somatic cells, as well as two unexpected somatic cell types. Our analysis revealed a continuous developmental trajectory of germ cells from spermatogonia to spermatids and identified candidate transcriptional regulators at several transition points during differentiation. Focused analyses delineated four subtypes of spermatogonia and nine subtypes of Sertoli cells; the latter linked to histologically defined developmental stages over the seminiferous epithelial cycle. Overall, this high-resolution cellular atlas represents a community resource and foundation of knowledge to study germ cell development and in vivo gametogenesis.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Triton X-100, laboratory grade
Sigma-Aldrich
Triton X-100, for molecular biology
Sigma-Aldrich
L-Arginine-13C6,15N4,2,3,3,4,4,5,5-d7 hydrochloride, 99 atom % 13C, 98 atom % 15N, 98 atom % D, 95% (CP)
Sigma-Aldrich
Ficoll® solution, Type 400, 20% in H2O
Sigma-Aldrich
1H,1H,2H,2H-Perfluoro-1-octanol, 97%