B4639

Biotin

powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%

• Synonym(s): D-Biotin, Bios II, Coenzyme R, Vitamin B7, Vitamin H
• Empirical Formula (Hill Notation): C₁₀H₁₆N₂O₃S
• CAS Number: 58-85-5
• Molecular Weight: 244.31
• Beilstein: 86838
• EC Number: 200-399-3
• MDL number: MFCD00005541
• UNSPSC Code: 12352201
• eCl@ss: 34058011
• PubChem Substance ID: 24891795
• NACRES: NA.21

Properties

• product line: BioReagent
• Assay: ≥99%
• form: powder
• mol wt: M_w 244.31 g/mol
• technique(s): cell culture | insect: suitable; cell culture | mammalian: suitable; cell culture | plant: suitable
• color: white to off-white
• mp: 231-233 °C (lit.)
• solubility: H₂O: 0.2 mg/mL (Solubility increases with addition of 1 N NaOH.)
• storage temp.: 2-8°C
• SMILES string: [H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2
• InChI: 1S/C10H16N2O3S/c13-8(14)4-2-1-3-7-9-6(5-16-7)11-10(15)12-9/h6-7,9H,1-5H2,(H,13,14)(H2,11,12,15)/t6-,7-,9-/m0/s1
• InChI key: YBJHBAHKTGYVGT-ZKWXMUAHSA-N

Description

General description

Biotin is also called as B complex vitamin. It is a bicyclic compound, which has a molecular weight of 244.31 Da. Biotin serves as an important cofactor for mammalian carboxylases. It is found within the cells in free and bound pools.

Application

Biotin has been used:

• as a M199 media supplement in in vitro culture system
• as a component of SATO serum free medium for the isolation of cortical oligodendrocyte precursors (OPCs)
• in biochemical precursors culture for de novo fatty acid synthesis
• as a component in aggregate culture media

Biochem/physiol Actions

Essential vitamin that is important for amino acid and energy metabolism, and fatty acid synthesis. It is a prosthetic group in four mammalian carboxylase families and facilitates the binding and transfer of carbon dioxide.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves