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Key Documents

566661

Sigma-Aldrich

SAG

≥95% (HPLC), liquid, smoothened agonist, Calbiochem®

Synonym(s):

InSolution Smoothened Agonist, SAG, SAG 1.3 in Solution, SAG1.3 in Solution

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About This Item

Empirical Formula (Hill Notation):
C28H28ClN3OS · 2HCl · xH2O
Molecular Weight:
562.98 (anhydrous basis)
UNSPSC Code:
12352200
NACRES:
NA.77

product name

Smoothened Agonist, SAG, InSolution, ≥95%, 10 mM aqueous solution

Quality Level

100

Assay

≥95% (HPLC)

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

shipped in

wet ice

storage temp.

−20°C

General description

A cell-permeable benzothiophene compound that modulates the coupling of Smo with its downstream effector by interacting with the Smo heptahelical domain (KD = 59 nM). Shown to induce Hedgehog pathway activation (EC50 ∼3 nM in NIH 3T3-derived Shh-LIGHT2 cells) and counteracts Cyclopamine-KAAD (Cat. No. 239804) inhibition of Smo. Reported to act as an activator at low concentrations and as an inhibitor at very high concentrations.

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Physical form

A 10 mM (500 µg/86 µl) solution of Smoothened Agonist, SAG (Cat. No. 566660) in H₂O.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Meloni, A.R., et al. 2006. Mol. Cell. Biol.26, 7750.
Chen, W., et al. 2004. Science306, 2257.
Chen, J.K., et al. 2002. Proc. Natl. Acad. Sci. USA99, 14071.
Frank-Kamenetsky, M., et al. 2002. J. Biol.1, 10.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The EMBO journal, 40(2), e105499-e105499 (2020-11-27)
Cilia of higher animals sense various environmental stimuli. Proper ciliary signaling requires appropriate extent of BBSome-mediated export of membrane receptors across ciliary barrier transition zone (TZ) through retrograde intraflagellar transport (IFT) machinery. How the barrier passage is controlled, however, remains
Sumeda Nandadasa et al.
Nature communications, 10(1), 953-953 (2019-03-01)
Although hundreds of cytosolic or transmembrane molecules form the primary cilium, few secreted molecules are known to contribute to ciliogenesis. Here, homologous secreted metalloproteases ADAMTS9 and ADAMTS20 are identified as ciliogenesis regulators that act intracellularly. Secreted and furin-processed ADAMTS9 bound
Koji Nakade et al.
Cell reports methods, 3(12), 100662-100662 (2023-12-13)
Although recent advances in genome editing technology with homology-directed repair have enabled the insertion of various reporter genes into the genome of mammalian cells, the efficiency is still low due to the random insertion of donor vectors into the host
Overexpression screen of chromosome 21 genes reveals modulators of Sonic hedgehog signaling relevant to Down syndrome.
Moyer, et al.
Disease models & mechanisms, 16 (2023)
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International journal of molecular sciences, 23(6) (2022-03-26)
Restoring the control of food intake is the key to obesity management and prevention. The arcuate nucleus (ARC) of the hypothalamus is extensively being studied as a potential anti-obesity target. Animal studies showed that neuropeptide FF (NPFF) reduces food intake
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