Skip to Content
Merck
  • Influence of physico-chemical parameters of some barbituric acid derivatives on their retention on an amide embedded RP silica column.

Influence of physico-chemical parameters of some barbituric acid derivatives on their retention on an amide embedded RP silica column.

Journal of pharmaceutical and biomedical analysis (2002-02-12)
Annamaria Jakab, Miklos Prodan, Esther Forgács
ABSTRACT

Retention parameters of 45 different barbituric acid derivatives were determined on an amide embedded RP silica column (Discovery RP-AmideC16) using non-buffered water-acetonitrile eluent systems. Linear correlation were calculated between the logarithm of the capacity factor and the acetonitrile concentration in the eluent. To determine the retention behavior of barbituric acid derivatives, stepwise regression analysis (SRA) and principal component analysis (PCA) followed by two-dimensional nonlinear and modified nonlinear mapping was used. It can be concluded, the retention of barbituric acid derivatives are governed mainly by the steric parameters of the substituents. Principal component analysis indicated that the barbituric acid derivatives have mixed retention on this amide embedded RP silica column in water-acetonitrile eluent.

MATERIALS
Product Number
Brand
Product Description

Supelco
Discovery® RP-AmideC16 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 3 mm
Supelco
Discovery® RP-AmideC16 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 3 mm
Supelco
Discovery® RP-AmideC16 Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm, pkg of 2 ea
Supelco
Discovery® RP-AmideC16 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
Supelco
Discovery® RP-AmideC16 Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 3 mm
Supelco
Discovery® RP-AmideC16 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4 mm
Supelco
Discovery® RP-AmideC16 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
Supelco
Discovery® RP-AmideC16 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4 mm
Supelco
Discovery® RP-AmideC16 HPLC Column, 5 μm particle size, L × I.D. 12.5 cm × 4 mm
Supelco
Discovery® RP-AmideC16 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 2.1 mm
Supelco
Discovery® RP-AmideC16 Supelguard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm, pkg of 1 kit
Supelco
Discovery® RP-AmideC16 HPLC Column, 5 μm particle size, L × I.D. 5 cm × 4.6 mm