- Polymerase chain reaction detection of bacterial 16S rRNA gene in human blood.
Polymerase chain reaction detection of bacterial 16S rRNA gene in human blood.
Microbiology and immunology (2008-08-01)
Kosei Moriyama, Chie Ando, Kosuke Tashiro, Satoru Kuhara, Seiichi Okamura, Shuji Nakano, Yasumitsu Takagi, Takeyoshi Miki, Yoshiyuki Nakashima, Hideki Hirakawa
PMID18667036
ABSTRACT
Bacterial 16S ribosomal RNA genes (rDNA) were detected in blood samples from two healthy individuals by PCR under conditions involving 30 cycles that did not produce any visible products from negative control saline. Even from control samples, PCR involving 35-40 cycles yielded visible bands. Major clones detected in the blood samples, but not in control, were the Aquabacterium subgroup, Stenotrophomonas subgroup, Budvicia subgroup, Serratia subgroup, Bacillus subgroup and Flavobacteria subgroup. No clone was located within the bacteroides-clostridium-lactobacillus cluster, which is indigenous to gastrointestinal flora.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
REDExtract-N-Amp™ Blood PCR Kit, sufficient for 10 extractions, sufficient for 10 amplifications
Sigma-Aldrich
REDExtract-N-Amp™ Blood PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications
Sigma-Aldrich
REDExtract-N-Amp™ Blood PCR Kit, sufficient for 100 extractions, sufficient for 500 amplifications
Sigma-Aldrich
REDExtract-N-Amp™ Blood PCR Kit, sufficient for 1000 extractions, sufficient for 1000 amplifications