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  • PI4P and PI(4,5)P2 are essential but independent lipid determinants of membrane identity.

PI4P and PI(4,5)P2 are essential but independent lipid determinants of membrane identity.

Science (New York, N.Y.) (2012-06-23)
Gerald R V Hammond, Michael J Fischer, Karen E Anderson, Jon Holdich, Ardita Koteci, Tamas Balla, Robin F Irvine
ABSTRACT

The quantitatively minor phospholipid phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P(2)] fulfills many cellular functions in the plasma membrane (PM), whereas its synthetic precursor, phosphatidylinositol 4-phosphate (PI4P), has no assigned PM roles apart from PI(4,5)P(2) synthesis. We used a combination of pharmacological and chemical genetic approaches to probe the function of PM PI4P, most of which was not required for the synthesis or functions of PI(4,5)P(2). However, depletion of both lipids was required to prevent PM targeting of proteins that interact with acidic lipids or activation of the transient receptor potential vanilloid 1 cation channel. Therefore, PI4P contributes to the pool of polyanionic lipids that define plasma membrane identity and to some functions previously attributed specifically to PI(4,5)P(2), which may be fulfilled by a more general polyanionic lipid requirement.

MATERIALS
Product Number
Brand
Product Description

Avanti
18:1 PI(3,4)P2, 1,2-dioleoyl-sn-glycero-3-phospho-(1′-myo-inositol-3′,4′-bisphosphate) (ammonium salt), powder
Avanti
18:1 PI(3,5)P2, 1,2-dioleoyl-sn-glycero-3-phospho-(1′-myo-inositol-3′,5′-bisphosphate) (ammonium salt), powder
Avanti
08:0 PI(3,5)P2, 1,2-dioctanoyl-sn-glycero-3-phospho-(1′-myo-inositol-3′,5′-bisphosphate) (ammonium salt), powder
Avanti
18:1 PI(3,4,5)P3, 1,2-dioleoyl-sn-glycero-3-phospho-(1′-myo-inositol-3′,4′,5′-trisphosphate) (ammonium salt), powder
Avanti
08:0 PI(3,4,5)P3, 1,2-dioctanoyl-sn-glycero-3-phospho-(1′-myo-inositol-3′,4′,5′-trisphosphate) (ammonium salt), powder
Avanti
06:0 PI(3,4,5)P3, 1,2-dihexanoyl-sn-glycero-3-phospho-(1′-myo-inositol-3′,4′,5′-trisphosphate) (ammonium salt), powder
Avanti
06:0 PI(3,5)P2, 1,2-dihexanoyl-sn-glycero-3-phospho-(1′-myo-inositol-3′,5′-bisphosphate) (ammonium salt), powder
Avanti
18:1 PI(5)P, 1,2-dioleoyl-sn-glycero-3-phospho-(1′-myo-inositol-5′-phosphate) (ammonium salt), powder
Avanti
08:0 PI(4)P, Avanti Research - A Croda Brand
Avanti
18:0-20:4 PI(4,5)P2, 1-stearoyl-2-arachidonoyl-sn-glycero-3-phospho-(1′-myo-inositol-4′,5′-bisphosphate) (ammonium salt), powder
Avanti
08:0 PI(3,4)P2, 1,2-dioctanoyl-sn-glycero-3-phospho-(1′-myo-inositol-3′,4′-bisphosphate) (ammonium salt), powder
Avanti
08:0 PI(4,5)P2, Avanti Research - A Croda Brand
Avanti
18:0-20:4 PI(3,5)P2, 1-stearoyl-2-arachidonoyl-sn-glycero-3-phospho-(1′-myo-inositol-3′,5′-bisphosphate) (ammonium salt), powder
Avanti
18:1 PI(3)P, Avanti Research - A Croda Brand
Avanti
TopFluor PI(3,5)P2, Avanti Research - A Croda Brand 810186P, powder