G003764

HPLC Analysis of Furosemide (Lasix) in Horse Serum on Discovery^® C18 after SPE using Discovery^® DSC-18

application for SPE, application for HPLC

Properties

• technique(s): HPLC: suitable; solid phase extraction (SPE): suitable
• test parameters: sample preparation: SPE (Solid Phase Extraction); sample/matrix: acidified horse serum spiked with indapamide at 10 mg/mL; SPE tube/cartridge: Discovery DSC-18, 50 mg/1 mL (52601-U); condition: 1 mL methanol; 1 mL 10 mM potassium phosphate, pH 3; sample addition: 1 mL; washing: 10 mM potassium phosphate, pH 3; elution: 1 mL 60% methanol in DI water.; column: Discovery C18, 15 cm x 4.6 mm I.D., 5 μm particles (504955); mobile phase: [A] 10 mM KH₂PO₄, pH 3 (adjusted with H₃PO₄): [B] acetonitrile; (60:40, A:B); flow rate: 1 mL/min; column temp.: 35 °C; detector: UV, 234 nm; injection: 10 μL
• suitability: application for HPLC; application for SPE
• application(s): forensics and toxicology

Description

Application

Sample Pre-treatment:
Neat horse serum was acidified with 10 mL 6 M HCl/mL serum. Internal standard (I.S.) blank was prepared by spiking acidified horse serum with indapamide at 10 mg/mL. Sample A = 10 mg/mL furosemide in I.S. blank. Sample B = 5 mg/mL furosemide in I.S. blank. Sample C = 0.5 mg/mL furosemide in I.S. blank. Sample D = 0.1 mg/mL furosemide in I.S. blank. Sample E = 0.05 mg/mL furosemide in I.S. blank.

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany