T5694

TRAIL human

recombinant, expressed in NSO cells, >97% (SDS-PAGE), lyophilized powder

• Synonym(s): Apo2 Ligand, Apo2L, TL2, TNF-related apoptosis-inducing ligand
• CAS Number: 2244730-93-4
• MDL number: MFCD01867158
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• Quality Level: 200
• recombinant: expressed in NSO cells
• description: N-terminal 6X histidine-tagged extracellular domain
• Assay: >97% (SDS-PAGE)
• form: lyophilized powder
• mol wt: 24 kDa by SDS-PAGE; predicted mol wt ~21 kDa
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• UniProt accession no.: P50591
• storage temp.: −20°C
• Gene Information: human ... TNFSF10(8743)

Description

Application

TRAIL human was used to induce cytotoxicity in A549, a human lung carcinoma cell line.

Biochem/physiol Actions

TNF-related apoptosis-inducing ligand (TRAIL) is a type II transmembrane protein that belongs to TNF family. It is present in both soluble as well as membrane-bound form and can induce rapid apoptosis of various transformed cell lines but not the normal cells. Additionally, the product can also induces apoptotic cell death in TRAIL-sensitive U343MGcells.

Type II transmembrane protein of the TNF superfamily. Ligand for apoptosis-inducing death receptors DR4 and DR5, decoy receptors DcR1 and DcR2, and osteoprotegerin. Approximately 28% amino acid sequence identity to FAS ligand. Human and mouse TRAIL share 65% sequence identity.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.

Analysis Note

Induces cytotoxicity in mouse L-929 cells in the presence of actinomycin D.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable